MAGneat gDNA Extraction Kit
£200.00
Description
Extracts high molecular weight DNA from blood, tissue, saliva, bacterial cells, buffy coat, buccal swabs and amniotic fluid. Suitable for PCR, qPCR, RT-qPCR, NGS, Sanger Sequencing, HRM and Genotyping.
Overview of kit:
MAGneat gDNA Kit is a magnetic bead-based genomic DNA extraction kit designed for rapid purification of high-quality genomic DNA from biological samples for PCR, qPCR, NGS, Sanger Sequencing, HRM and other molecular biology workflows.
Principle of kit summary:
The workflow begins with cell lysis to release genomic DNA from the sample. The DNA then binds to magnetic beads, allowing it to be separated from proteins, salts and other contaminants. A series of wash steps removes remaining impurities while the DNA remains bound to the beads. Finally, purified genomic DNA is eluted into a low-salt buffer, producing high-quality DNA suitable for downstream applications such as PCR, qPCR, sequencing and genotyping.
Additional information
| Quantity | 96 preps |
|---|
Extracts high molecular weight DNA from blood, tissue, saliva, bacterial cells, buffy coat, buccal swabs and amniotic fluid. Suitable for PCR, qPCR, RT-qPCR, NGS, Sanger Sequencing, HRM and Genotyping.
Overview of kit:
MAGneat gDNA Kit is a magnetic bead-based genomic DNA extraction kit designed for rapid purification of high-quality genomic DNA from biological samples for PCR, qPCR, NGS, Sanger Sequencing, HRM and other molecular biology workflows.
Principle of kit summary:
The workflow begins with cell lysis to release genomic DNA from the sample. The DNA then binds to magnetic beads, allowing it to be separated from proteins, salts and other contaminants. A series of wash steps removes remaining impurities while the DNA remains bound to the beads. Finally, purified genomic DNA is eluted into a low-salt buffer, producing high-quality DNA suitable for downstream applications such as PCR, qPCR, sequencing and genotyping.
Figure 1. gDNA was extracted from whole blood collected in sodium citrate, using the MAGneat gDNA Extraction Kit. The extracted DNA was analysed using the Tapestation (Agilent, Santa Clara), fragment lengths of >60 Kb and DNA integrity numbers (DIN) of 9.5-9.6 were observed.
Figure 2: High-Molecular-Weight genomic DNA extracted from different tissue types with MAGneat GDNA Extraction Kit. Agarose gel electrophoresis shows high quality genomic DNA extracted from avian muscle, porcine kidney, caprine heart and porcine liver with MAGneat gDNA Extraction Kit. M = 1 kb ladder
Figure 3: Highly pure genomic DNA extracted from fresh saliva with MAGneat gDNA Extraction Kit. Genomic DNA was extracted with MAGneat gDNA Extraction Kit from human fresh saliva samples obtained from five different donors. Analysis was carried out using the NP80 Touch Nanophotometer (Implen – Germany) to determine DNA concentration, A260/A280 & A260/230 ratios.
Figure 4: DNA Yield Comparison of Fresh and Frozen Whole Blood Samples obtained in Sodium Citrate, EDTA & Sodium Heparin collection tubes. Whole blood samples were collected from 3 different donors in 3 collection tubes: sodium citrate, EDTA and sodium heparin. DNA extraction was performed in triplicates with MAGneat gDNA Extraction Kit and DNA concentration was measured using the Qubit 4 Fluorometer (ThermoFisher – USA).
Figure 5: High-molecular weight genomic DNA from both Gram-Positive and Gram-Negative bacteria extracted with MAGneat gDNA extraction kit. Bacterial genomic DNA was extracted from Staphylococcus aureus, Acinetobacter baumannii, Escherichia coli and Pseudomonas aeruginosa using MAGneat gDNA extraction kit. 5 µL of the eluted DNA was loaded to 1% agarose gel for DNA analysis. M = 1 kb DNA ladder.
Figure 6: DNA yield comparison from a range of bacteria species extracted using the MAGneat gDNA Extraction Kit and a comparator N. DNA from 50 mg (wet weight) of both gram-positive bacteria (Staphylococcus aureus) and gram-negative bacteria (Acinetobacter baumannii, Escherichia coli and Pseudomonas aeruginosa) cells was extracted with MAGneat gDNA extraction kit and a comparator. DNA concentration was measured using the Qubit 4 Fluorometer from ThermoFisher.
Compatible Sample Types
This kit is compatible with the following sample types:
- EDTA Blood
- Heparin Blood
- Citrate Blood
- Animal Tissue
- Stabilised Saliva
- Fresh Saliva
- Bacterial Culture
- Buffy Coat
- Bucccal Cells
- Amniotic Fluid
Workflow summary:
Lysis → DNA binding to magnetic beads → Washing → RNase treatment (optional) → DNA Elution → Downstream processing
- High molecular weight DNA recovery
- Magnetic bead-based technology
- Compatible with multiple sample types
- 45 minute workflow
- Automation ready
- High purity DNA
- Reliable DNA integrity
- Consistent and reproducible results
- PCR and qPCR workflows
- Next Generation Sequencing (NGS)
- Micro-array Genotyping
- Enzymatic reactions
- Sanger Sequencing
- High molecular weight genomic DNA
- Product name: MAGneat gDNA Extraction Kit
- Brand: Microzone
- SKU: 2MN-BTS-96
-
UNSPSC Code: 41105504
- Description: magnetic bead-based gDNA extraction kit designed to purify high-quality, high molecular weight genomic DNA from blood, tissue, saliva and bacterial samples suitable for downstream molecular biology applications.
- Image: updated image of kit
- Availability: same day shipping before noon
- Price: £200
- Category: Extraction
- Technology: magnetic bead
- DNA type: genomic
- Extraction time: 45 minutes
- Automation compatible: Yes
- Sample types: whole blood, tissue, saliva, bacterial cultures, buffy coat, buccal swabs and amniotic fluid.
What is a magnetic bead DNA extraction kit?
Answer: A magnetic bead DNA extraction kit uses magnetic particles that selectively and reversibly bind DNA. A magnet is then used to separate the DNA-bound beads from the sample, allowing contaminants to be removed during wash steps before the purified DNA is eluted. MAGneat combines proprietary buffers with MAGneat magnetic beads to isolate high-molecular-weight DNA.
What is genomic DNA extraction?
Answer: Genomic DNA extraction is the process of isolating and purifying DNA from cells or tissues while removing proteins, RNA, and other contaminants. The MAGneat gDNA Extraction Kit is designed to recover high-molecular-weight genomic DNA, with fragment lengths greater than 60 kb, suitable for applications including PCR, NGS, and enzymatic workflows.
What samples can this kit process?
Answer: Whole blood (EDTA, Heparin, Citrate), saliva (fresh and stabilised), tissue samples (animal and human) and bacteria (Gram‑positive & Gram‑negative), buffy coat, buccal swabs and amniotic fluid.
How much starting material is required?
Answer:
Blood: 200 µL (EDTA, Heparin, Citrate)
Bacteria: up to 50 mg
Tissue: up to 50 mg
Stabilised Saliva 500 µL
Fresh Saliva 200 µL
Buffy coat 100 µL
Swab 1 swab
Amniotic Fluid 1 – 5 mL
Is the kit suitable for PCR?
Answer: Yes. The kit produces high‑purity DNA suitable for PCR and other sensitive enzymatic applications.
Can extracted DNA be used for sequencing?
Answer: Yes. The kit yields high molecular weight DNA appropriate for next generation sequencing (NGS) and sanger sequencing workflows.
Can the workflow be automated?
Answer: Yes. MAGneat is designed for automation and has been validated across multiple magnetic bead-based extraction platforms, including the KingFisher® Flex System and the HeiDi-NA MAGneat Nucleic Acid Extraction/Purification System. The HeiDi-NA processes up to 16 samples per run.
How does magnetic bead extraction compare with spin columns?
Answer: Magnetic bead extraction typically offers greater binding capacity and applies less shear stress to high-molecular-weight (HMW) DNA than traditional spin columns. This can result in higher DNA yields and better recovery of longer, more intact DNA fragments.
What DNA purity can be expected?
Answer: The MAGneat gDNA Extraction Kit yields high-purity, high-quality DNA, with typical A260/A280 ratios of 1.75–1.85 and A260/A230 ratios of 1.7–2.2, as measured by NanoPhotometer. Extracted DNA also demonstrates excellent integrity, with DIN values of 9.5–9.6 and fragment lengths greater than 60 kb. Determined by TapeStation®.
Is RNase treatment required?
Answer: RNase treatment is optional but recommended when RNA contamination may affect downstream applications. It removes residual RNA, improving DNA purity and supporting more accurate quantification and reliable performance in applications such as PCR and sequencing.
What downstream applications are supported?
Answer: PCR, qPCR, NGS, Sanger Sequencing, Cloning, Enzymatic reactions, HRM, ONT (Oxford Nanopore Technologies), Long‑read workflows (high molecular weight DNA).
How long does the extraction take?
Answer: DNA extraction can be completed in as little as 45 minutes.
Can the kit process difficult tissue samples?
Answer: Yes. The kit has demonstrated strong performance with challenging tissue types, including avian muscle, porcine kidney, caprine heart, and porcine liver, including fibrous and inhibitor-rich samples.
What equipment is required?
Answer: The MAGneat gDNA Extraction Kit requires a magnetic rack or compatible automated magnetic processor, such as the HeiDi-NA, along with standard laboratory equipment including pipettes, filter tips, 2 mL microcentrifuge tubes, and a vortex mixer. A heating block and centrifuge may also be required depending on the sample type and protocol.
What additional reagents are required?
Answer: 100% Isopropanol (IPA) and 100% ethanol are required for the extraction workflow. RNase A is optional and may be used when RNA removal is required.
How should the kit be stored?
Answer: All kit components can be stored at room temperature, except the MAGneat magnetic beads, which should be stored at 2–8°C to help minimise the risk of microbial contamination.
Does the kit remove PCR inhibitors?
Answer: Yes. The MAGneat gDNA Extraction Kit uses an optimised buffer system to remove contaminants and PCR inhibitors, producing high-purity DNA suitable for sensitive downstream applications such as PCR and sequencing.
Can multiple samples be processed at once?
Answer: Yes. The workflow supports parallel processing, either manually or through automation. The HeiDi-NA can handle up to 16 samples per run, while higher-throughput platforms such as the KingFisher can process up to 96 samples per run.
Is the kit labelled for IVD?
Answer: No. The MAGneat gDNA Extraction Kit is labelled Research Use Only (RUO).
What elution volume is recommended according to sample type?
Answer: Recommended elution volumes range from 50–400 µL, depending on the sample type and desired DNA concentration. Refer to the IFU for sample-specific recommendations.
- High molecular weight DNA recovery
- Magnetic bead-based technology
- Compatible with multiple sample types
- 45 minute workflow
- Automation ready
- High purity DNA
- Reliable DNA integrity
- Consistent and reproducible results
- PCR and qPCR workflows
- Next Generation Sequencing (NGS)
- Micro-array Genotyping
- Enzymatic reactions
- Sanger Sequencing
- High molecular weight genomic DNA
- Product name: MAGneat gDNA Extraction Kit
- Brand: Microzone
- SKU: 2MN-BTS-96
-
UNSPSC Code: 41105504
- Description: magnetic bead-based gDNA extraction kit designed to purify high-quality, high molecular weight genomic DNA from blood, tissue, saliva and bacterial samples suitable for downstream molecular biology applications.
- Image: updated image of kit
- Availability: same day shipping before noon
- Price: £200
- Category: Extraction
- Technology: magnetic bead
- DNA type: genomic
- Extraction time: 45 minutes
- Automation compatible: Yes
- Sample types: whole blood, tissue, saliva, bacterial cultures, buffy coat, buccal swabs and amniotic fluid.
What is a magnetic bead DNA extraction kit?
Answer: A magnetic bead DNA extraction kit uses magnetic particles that selectively and reversibly bind DNA. A magnet is then used to separate the DNA-bound beads from the sample, allowing contaminants to be removed during wash steps before the purified DNA is eluted. MAGneat combines proprietary buffers with MAGneat magnetic beads to isolate high-molecular-weight DNA.
What is genomic DNA extraction?
Answer: Genomic DNA extraction is the process of isolating and purifying DNA from cells or tissues while removing proteins, RNA, and other contaminants. The MAGneat gDNA Extraction Kit is designed to recover high-molecular-weight genomic DNA, with fragment lengths greater than 60 kb, suitable for applications including PCR, NGS, and enzymatic workflows.
What samples can this kit process?
Answer: Whole blood (EDTA, Heparin, Citrate), saliva (fresh and stabilised), tissue samples (animal and human) and bacteria (Gram‑positive & Gram‑negative), buffy coat, buccal swabs and amniotic fluid.
How much starting material is required?
Answer:
Blood: 200 µL (EDTA, Heparin, Citrate)
Bacteria: up to 50 mg
Tissue: up to 50 mg
Stabilised Saliva 500 µL
Fresh Saliva 200 µL
Buffy coat 100 µL
Swab 1 swab
Amniotic Fluid 1 – 5 mL
Is the kit suitable for PCR?
Answer: Yes. The kit produces high‑purity DNA suitable for PCR and other sensitive enzymatic applications.
Can extracted DNA be used for sequencing?
Answer: Yes. The kit yields high molecular weight DNA appropriate for next generation sequencing (NGS) and sanger sequencing workflows.
Can the workflow be automated?
Answer: Yes. MAGneat is designed for automation and has been validated across multiple magnetic bead-based extraction platforms, including the KingFisher® Flex System and the HeiDi-NA MAGneat Nucleic Acid Extraction/Purification System. The HeiDi-NA processes up to 16 samples per run.
How does magnetic bead extraction compare with spin columns?
Answer: Magnetic bead extraction typically offers greater binding capacity and applies less shear stress to high-molecular-weight (HMW) DNA than traditional spin columns. This can result in higher DNA yields and better recovery of longer, more intact DNA fragments.
What DNA purity can be expected?
Answer: The MAGneat gDNA Extraction Kit yields high-purity, high-quality DNA, with typical A260/A280 ratios of 1.75–1.85 and A260/A230 ratios of 1.7–2.2, as measured by NanoPhotometer. Extracted DNA also demonstrates excellent integrity, with DIN values of 9.5–9.6 and fragment lengths greater than 60 kb. Determined by TapeStation®.
Is RNase treatment required?
Answer: RNase treatment is optional but recommended when RNA contamination may affect downstream applications. It removes residual RNA, improving DNA purity and supporting more accurate quantification and reliable performance in applications such as PCR and sequencing.
What downstream applications are supported?
Answer: PCR, qPCR, NGS, Sanger Sequencing, Cloning, Enzymatic reactions, HRM, ONT (Oxford Nanopore Technologies), Long‑read workflows (high molecular weight DNA).
How long does the extraction take?
Answer: DNA extraction can be completed in as little as 45 minutes.
Can the kit process difficult tissue samples?
Answer: Yes. The kit has demonstrated strong performance with challenging tissue types, including avian muscle, porcine kidney, caprine heart, and porcine liver, including fibrous and inhibitor-rich samples.
What equipment is required?
Answer: The MAGneat gDNA Extraction Kit requires a magnetic rack or compatible automated magnetic processor, such as the HeiDi-NA, along with standard laboratory equipment including pipettes, filter tips, 2 mL microcentrifuge tubes, and a vortex mixer. A heating block and centrifuge may also be required depending on the sample type and protocol.
What additional reagents are required?
Answer: 100% Isopropanol (IPA) and 100% ethanol are required for the extraction workflow. RNase A is optional and may be used when RNA removal is required.
How should the kit be stored?
Answer: All kit components can be stored at room temperature, except the MAGneat magnetic beads, which should be stored at 2–8°C to help minimise the risk of microbial contamination.
Does the kit remove PCR inhibitors?
Answer: Yes. The MAGneat gDNA Extraction Kit uses an optimised buffer system to remove contaminants and PCR inhibitors, producing high-purity DNA suitable for sensitive downstream applications such as PCR and sequencing.
Can multiple samples be processed at once?
Answer: Yes. The workflow supports parallel processing, either manually or through automation. The HeiDi-NA can handle up to 16 samples per run, while higher-throughput platforms such as the KingFisher can process up to 96 samples per run.
Is the kit labelled for IVD?
Answer: No. The MAGneat gDNA Extraction Kit is labelled Research Use Only (RUO).
What elution volume is recommended according to sample type?
Answer: Recommended elution volumes range from 50–400 µL, depending on the sample type and desired DNA concentration. Refer to the IFU for sample-specific recommendations.
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