MyZol for TRIzol – MAGneat RNA Extraction Kit
£175.00
Description
Extracts high molecular weight RNA from cell cultures, animal tissues, viral samples, biological fluids and TRIzol™ preserved samples. Suitable for RT-qPCR, sequencing, gene expression studies and infectious disease research.
Overview of kit:
The MyZol for TRIzol™ Kit enables rapid, high-quality RNA purification from TRIzol-preserved samples using advanced magnetic bead technology. It delivers reliable RNA yields and supports both manual and automated workflows for consistent downstream molecular applications.
Principle of kit summary:
MyZol for TRIzol – MAGneat RNA Extraction Kit uses magnetic bead technology to efficiently isolate and purify high-quality RNA from samples preserved in TRIzol™ reagent, including cells, tissues, viruses, and biological fluids. Following TRIzol-based sample preparation, RNA selectively and reversibly binds to MAGneat magnetic beads, allowing efficient removal of contaminants through optimised washing steps. The purified RNA is then eluted to provide high-yield, high-purity material suitable for sensitive downstream applications including RT-PCR, RT-qPCR, RNA sequencing (RNA-seq), microarray analysis, and enzymatic reactions. The workflow simplifies TRIzol RNA purification, eliminates the need for traditional phase separation steps, and is compatible with manual and automated extraction platforms.
Additional information
| Quantity | 50 Extractions |
|---|
Extracts high molecular weight RNA from cell cultures, animal tissues, viral samples, biological fluids and TRIzol™ preserved samples. Suitable for RT-qPCR, sequencing, gene expression studies and infectious disease research.
Overview of kit:
The MyZol for TRIzol™ Kit enables rapid, high-quality RNA purification from TRIzol-preserved samples using advanced magnetic bead technology. It delivers reliable RNA yields and supports both manual and automated workflows for consistent downstream molecular applications.
Principle of kit summary:
MyZol for TRIzol – MAGneat RNA Extraction Kit uses magnetic bead technology to efficiently isolate and purify high-quality RNA from samples preserved in TRIzol™ reagent, including cells, tissues, viruses, and biological fluids. Following TRIzol-based sample preparation, RNA selectively and reversibly binds to MAGneat magnetic beads, allowing efficient removal of contaminants through optimised washing steps. The purified RNA is then eluted to provide high-yield, high-purity material suitable for sensitive downstream applications including RT-PCR, RT-qPCR, RNA sequencing (RNA-seq), microarray analysis, and enzymatic reactions. The workflow simplifies TRIzol RNA purification, eliminates the need for traditional phase separation steps, and is compatible with manual and automated extraction platforms.
Table 1. MyZol for TRIzol — MAGneat RNA Extraction Kit outperforms other commercially available TRIzol RNA extraction kits. RNA was extracted from 6.25 mg of homogenised avian muscle tissue in TRIzolTM reagent, using the MyZol for TRIzol — MAGneat RNA Extraction Kit and a commercially available comparator. Yield was measured using the Qubit 4 Fluorimeter from ThermoFisher and the A260/A280 and A260/A230 ratios were assessed using the NP80 Touch Nanophotometer from Implen.
Figure 1. Comparison of Manual and Automated MyZol for TRIzol — MAGneat RNA Extraction Kit. RT-qPCR amplification of the RdRp gene from SARS-CoV-2 viral RNA. Nasopharyngeal swab was collected and stored in TRIzol reagent and extracted using the MyZol for TRIzol — MAGneat RNA Extraction Kit using both manual (MAGneat magnetic separator) and automated (HeiDi-NA automated extraction system) methods. Green curves represent manually extracted samples and blue curves represent samples extracted using the HeiDi-NA automated extraction system.
Compatible Sample Types
This kit is compatible with the following sample types:
-
- TRIzol™ preserved samples
- Cell cultures
- Viral Samples
- Saliva
- Biological fluids
Workflow summary:
Sample in TRIzol™ → RNA binding to magnetic beads → Washing → RNA Elution → Downstream processing.
• High molecular weight RNA recovery
• No phase separation required
• Magnetic bead-based technology
• Compatible with multiple sample types
• Automation ready
• High purity RNA
• Consistent and reproducible results
• Compatible with TRIzol™ preserved samples
- RNA extraction from TRIzol™ preserved samples
- Infectious disease research
- Gene expression studies
- RT-qPCR
- RNA sequencing (RNA-seq)
- High throughput laboratories
- Microarray genotyping
- Enzymatic Reactions
- Product name: MyZol for TRIzol™ – RNA Extraction Kit
- Brand: Microzone
- SKU: 2MN-MYR-50
- Description: magnetic bead-based TRIzol™ RNA extraction kit designed to purify high-quality, high molecular weight RNA from cell cultures, animal tissues, viral samples, biological fluids and TRIzol™ preserved samples for downstream molecular biology applications.
- Image: updated image of kit
- Availability: same day shipping before noon
- Price: £175
- Category: Extraction
- Technology: magnetic bead
- Nucleic Acid type: RNA
- Automation compatible: Yes
- Sample types: cell cultures, animal tissues, viral samples and biological samples
What is the MyZol for TRIzol™ – MAGneat RNA Extraction Kit?
Answer: The MyZol for TRIzol™ – MAGneat RNA Extraction Kit is a magnetic bead-based system designed to purify high-quality RNA from samples lysed or preserved in TRIzol™ reagent. The workflow eliminates the need for traditional chloroform phase separation and uses MAGneat magnetic bead technology to isolate purified RNA for downstream molecular biology applications.
What samples can be processed with MyZol?
Answer: MyZol is designed for samples lysed or preserved in TRIzol™ reagent, including cell cultures, tissue samples and other biological samples compatible with TRIzol™-based workflows.
What is TRIzol™ reagent?
Answer: TRIzol™ is a monophasic reagent used to lyse biological samples and protect RNA from degradation during sample processing. It is widely used as the first step in RNA extraction workflows from cells, tissues and other biological samples.
Why use magnetic beads instead of phase separation?
Answer: Magnetic bead purification simplifies RNA extraction by replacing traditional liquid-liquid phase separation with selective RNA binding, washing and elution. This reduces manual handling and provides a workflow that can be readily adapted to automated magnetic bead-based extraction platforms.
Does MyZol eliminate chloroform phase separation?
Answer: Yes. MyZol is designed to purify RNA from TRIzol™-treated samples without the traditional chloroform phase separation step, simplifying the extraction workflow and reducing hands-on processing.
Is the RNA suitable for RT-PCR?
Answer: Yes. The kit produces purified RNA suitable for reverse transcription PCR (RT-PCR) and other sensitive downstream enzymatic applications.
Does the kit produce high-quality RNA?
Answer: Yes. MyZol combines optimised buffer chemistry with MAGneat magnetic bead technology to efficiently purify RNA while removing contaminants that may interfere with downstream applications.
Can the RNA be used for RNA sequencing (NGS)?
Answer: Yes. Purified RNA is suitable for RNA sequencing (RNA-seq) and other Next Generation Sequencing (NGS) workflows following appropriate downstream sample preparation.
Can the RNA be used for microarray analysis?
Answer: Yes. Purified RNA is suitable for microarray-based gene expression analysis and other transcriptomic applications requiring high-quality RNA.
Can the RNA be used for enzymatic reactions?
Answer: Yes. Purified RNA is suitable for a range of enzymatic reactions and downstream molecular biology workflows.
Does TRIzol™ inactivate biological material?
Answer: TRIzol™ lyses cells and disrupts biological material during sample processing. However, appropriate laboratory biosafety procedures should always be followed, and users should not assume that TRIzol™ treatment alone renders all biological samples non-infectious.
Can the workflow be automated?
Answer: Yes. MyZol is designed for both manual and automated magnetic bead-based workflows, providing flexibility for different laboratory throughput requirements.
Which automation platforms are supported?
Answer: MyZol is designed for automation and has been validated across multiple magnetic bead-based extraction platforms, including the KingFisher® Purification System and the HeiDi-NA automated magnetic bead processing system. The HeiDi-NA processes up to 16 samples per run.
What equipment is required?
Answer: Typical equipment includes a magnetic rack or compatible automated magnetic processor, centrifuge, vortex mixer, air-displacement pipettes with appropriate filter tips, and compatible 2 mL microcentrifuge tubes.
What additional reagents are required?
Answer: Additional reagents include 100% ethanol, 100% isopropanol and molecular-grade water. Refer to the IFU for full reagent preparation and workflow requirements.
How long does the extraction take?
Answer: MyZol is designed for rapid RNA purification. Total extraction time is around 1 hour but may vary depending on sample preparation, processing method and whether the workflow is performed manually or using an automated platform.
How should the kit be stored?
Answer: The kits is stored between 2-8°C.
What downstream applications are supported?
Answer: Purified RNA is suitable for a range of downstream applications, including RT-PCR, RT-qPCR, RNA sequencing (RNA-seq), gene expression analysis, microarray analysis and other enzymatic molecular biology workflows.
How does MyZol compare with spin-column RNA extraction?
Answer: MyZol uses magnetic bead technology rather than spin columns, providing an automation-friendly workflow with reduced manual handling. Magnetic bead purification also enables efficient RNA recovery and straightforward scaling from manual to higher-throughput automated processing.
What makes MyZol different from traditional TRIzol™ extraction?
Answer: Unlike traditional TRIzol™ extraction workflows, MyZol eliminates the need for chloroform phase separation by using MAGneat magnetic bead technology to purify RNA directly from TRIzol™-treated samples. This simplifies processing, reduces hands-on handling and enables straightforward integration into automated extraction workflows.
Why choose the MyZol for TRIzol™ – MAGneat RNA Extraction Kit?
Answer: MyZol combines the sample lysis benefits of TRIzol™ with MAGneat magnetic bead purification in a simplified, automation-friendly workflow. By eliminating traditional chloroform phase separation, the kit reduces manual handling while producing purified RNA suitable for applications including RT-PCR, RT-qPCR, RNA sequencing and gene expression analysis.
• High molecular weight RNA recovery
• No phase separation required
• Magnetic bead-based technology
• Compatible with multiple sample types
• Automation ready
• High purity RNA
• Consistent and reproducible results
• Compatible with TRIzol™ preserved samples
- RNA extraction from TRIzol™ preserved samples
- Infectious disease research
- Gene expression studies
- RT-qPCR
- RNA sequencing (RNA-seq)
- High throughput laboratories
- Microarray genotyping
- Enzymatic Reactions
- Product name: MyZol for TRIzol™ – RNA Extraction Kit
- Brand: Microzone
- SKU: 2MN-MYR-50
- Description: magnetic bead-based TRIzol™ RNA extraction kit designed to purify high-quality, high molecular weight RNA from cell cultures, animal tissues, viral samples, biological fluids and TRIzol™ preserved samples for downstream molecular biology applications.
- Image: updated image of kit
- Availability: same day shipping before noon
- Price: £175
- Category: Extraction
- Technology: magnetic bead
- Nucleic Acid type: RNA
- Automation compatible: Yes
- Sample types: cell cultures, animal tissues, viral samples and biological samples
What is the MyZol for TRIzol™ – MAGneat RNA Extraction Kit?
Answer: The MyZol for TRIzol™ – MAGneat RNA Extraction Kit is a magnetic bead-based system designed to purify high-quality RNA from samples lysed or preserved in TRIzol™ reagent. The workflow eliminates the need for traditional chloroform phase separation and uses MAGneat magnetic bead technology to isolate purified RNA for downstream molecular biology applications.
What samples can be processed with MyZol?
Answer: MyZol is designed for samples lysed or preserved in TRIzol™ reagent, including cell cultures, tissue samples and other biological samples compatible with TRIzol™-based workflows.
What is TRIzol™ reagent?
Answer: TRIzol™ is a monophasic reagent used to lyse biological samples and protect RNA from degradation during sample processing. It is widely used as the first step in RNA extraction workflows from cells, tissues and other biological samples.
Why use magnetic beads instead of phase separation?
Answer: Magnetic bead purification simplifies RNA extraction by replacing traditional liquid-liquid phase separation with selective RNA binding, washing and elution. This reduces manual handling and provides a workflow that can be readily adapted to automated magnetic bead-based extraction platforms.
Does MyZol eliminate chloroform phase separation?
Answer: Yes. MyZol is designed to purify RNA from TRIzol™-treated samples without the traditional chloroform phase separation step, simplifying the extraction workflow and reducing hands-on processing.
Is the RNA suitable for RT-PCR?
Answer: Yes. The kit produces purified RNA suitable for reverse transcription PCR (RT-PCR) and other sensitive downstream enzymatic applications.
Does the kit produce high-quality RNA?
Answer: Yes. MyZol combines optimised buffer chemistry with MAGneat magnetic bead technology to efficiently purify RNA while removing contaminants that may interfere with downstream applications.
Can the RNA be used for RNA sequencing (NGS)?
Answer: Yes. Purified RNA is suitable for RNA sequencing (RNA-seq) and other Next Generation Sequencing (NGS) workflows following appropriate downstream sample preparation.
Can the RNA be used for microarray analysis?
Answer: Yes. Purified RNA is suitable for microarray-based gene expression analysis and other transcriptomic applications requiring high-quality RNA.
Can the RNA be used for enzymatic reactions?
Answer: Yes. Purified RNA is suitable for a range of enzymatic reactions and downstream molecular biology workflows.
Does TRIzol™ inactivate biological material?
Answer: TRIzol™ lyses cells and disrupts biological material during sample processing. However, appropriate laboratory biosafety procedures should always be followed, and users should not assume that TRIzol™ treatment alone renders all biological samples non-infectious.
Can the workflow be automated?
Answer: Yes. MyZol is designed for both manual and automated magnetic bead-based workflows, providing flexibility for different laboratory throughput requirements.
Which automation platforms are supported?
Answer: MyZol is designed for automation and has been validated across multiple magnetic bead-based extraction platforms, including the KingFisher® Purification System and the HeiDi-NA automated magnetic bead processing system. The HeiDi-NA processes up to 16 samples per run.
What equipment is required?
Answer: Typical equipment includes a magnetic rack or compatible automated magnetic processor, centrifuge, vortex mixer, air-displacement pipettes with appropriate filter tips, and compatible 2 mL microcentrifuge tubes.
What additional reagents are required?
Answer: Additional reagents include 100% ethanol, 100% isopropanol and molecular-grade water. Refer to the IFU for full reagent preparation and workflow requirements.
How long does the extraction take?
Answer: MyZol is designed for rapid RNA purification. Total extraction time is around 1 hour but may vary depending on sample preparation, processing method and whether the workflow is performed manually or using an automated platform.
How should the kit be stored?
Answer: The kits is stored between 2-8°C.
What downstream applications are supported?
Answer: Purified RNA is suitable for a range of downstream applications, including RT-PCR, RT-qPCR, RNA sequencing (RNA-seq), gene expression analysis, microarray analysis and other enzymatic molecular biology workflows.
How does MyZol compare with spin-column RNA extraction?
Answer: MyZol uses magnetic bead technology rather than spin columns, providing an automation-friendly workflow with reduced manual handling. Magnetic bead purification also enables efficient RNA recovery and straightforward scaling from manual to higher-throughput automated processing.
What makes MyZol different from traditional TRIzol™ extraction?
Answer: Unlike traditional TRIzol™ extraction workflows, MyZol eliminates the need for chloroform phase separation by using MAGneat magnetic bead technology to purify RNA directly from TRIzol™-treated samples. This simplifies processing, reduces hands-on handling and enables straightforward integration into automated extraction workflows.
Why choose the MyZol for TRIzol™ – MAGneat RNA Extraction Kit?
Answer: MyZol combines the sample lysis benefits of TRIzol™ with MAGneat magnetic bead purification in a simplified, automation-friendly workflow. By eliminating traditional chloroform phase separation, the kit reduces manual handling while producing purified RNA suitable for applications including RT-PCR, RT-qPCR, RNA sequencing and gene expression analysis.
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