MycoDetect – Mycoplasma qPCR Detection Kit
£495.00
Description
Rapid probe-based qPCR detection of common mycoplasma contaminants in cell cultures, with built-in internal and positive controls and results in less than one hour.
Overview of Kit:
MycoDetect is a rapid probe-based qPCR kit for detecting mycoplasma contamination in cell cultures. The assay targets a highly conserved 16S-rDNA region found in common Mollicutes associated with cell-culture contamination and provides results in less than one hour.
The kit includes an Internal Amplification Control (IAC) and positive control to help validate each qPCR run. Mycoplasma amplification is detected using the FAM channel, while the Internal Amplification Control is detected using HEX.
MycoDetect uses Microzone’s MegaMix Platinum Probe qPCR Mastermix containing Hot Start Taq, dUTP and UNG to help reduce false-positive results caused by PCR amplicon carryover contamination.
Principle of kit summary:
MycoDetect uses probe-based quantitative PCR to detect DNA from mycoplasma and other common Mollicutes associated with cell-culture contamination.
Primers and probes target a highly conserved 16S-rDNA region. During amplification, mycoplasma-specific DNA is detected through the FAM fluorescence channel, while an Internal Amplification Control (IAC) is detected through HEX.
The internal control helps confirm that the PCR reaction has performed correctly, while the supplied positive control provides confirmation that the mycoplasma detection chemistry is functioning.
The included MegaMix Platinum qPCR Mastermix contains dUTP and UNG, helping reduce false-positive results caused by carryover of previous PCR amplification products.
Additional information
| Quantity | 100 Reactions |
|---|
Rapid probe-based qPCR detection of common mycoplasma contaminants in cell cultures, with built-in internal and positive controls and results in less than one hour.
Overview of Kit:
MycoDetect is a rapid probe-based qPCR kit for detecting mycoplasma contamination in cell cultures. The assay targets a highly conserved 16S-rDNA region found in common Mollicutes associated with cell-culture contamination and provides results in less than one hour.
The kit includes an Internal Amplification Control (IAC) and positive control to help validate each qPCR run. Mycoplasma amplification is detected using the FAM channel, while the Internal Amplification Control is detected using HEX.
MycoDetect uses Microzone's MegaMix Platinum Probe qPCR Mastermix containing Hot Start Taq, dUTP and UNG to help reduce false-positive results caused by PCR amplicon carryover contamination.
Principle of kit summary:
MycoDetect uses probe-based quantitative PCR to detect DNA from mycoplasma and other common Mollicutes associated with cell-culture contamination.
Primers and probes target a highly conserved 16S-rDNA region. During amplification, mycoplasma-specific DNA is detected through the FAM fluorescence channel, while an Internal Amplification Control (IAC) is detected through HEX.
The internal control helps confirm that the PCR reaction has performed correctly, while the supplied positive control provides confirmation that the mycoplasma detection chemistry is functioning.
The included MegaMix Platinum qPCR Mastermix contains dUTP and UNG, helping reduce false-positive results caused by carryover of previous PCR amplification products.
Figure 1: One million copies of Metamycoplasma orale DNA detected with MycoDetect – Mycoplasma qPCR Detection Kit on FAM channel and Internal Amplification Control on HEX channel (inset).
Compatible Sample Types
This kit is compatible with the following sample types:
- Adherent cell-culture supernatant
- Suspension cell-culture supernatant
- Purified nucleic acids from cell-culture samples
- Cell-culture samples prepared using a suitable direct-to-PCR mycoplasma lysis method
Mycoplasma Species Detected include:
- Acholeplasma laidlawii
- Mycoplasma arginini
- Mycoplasma arthritidis
- Mycoplasma fermentans
- Mycoplasma gallisepticum
- Mycoplasma genitalium
- Mycoplasma hominis
- Mycoplasma hyorhinis
- Mycoplasma orale
- Mycoplasma penetrans
- Mycoplasma pneumoniae
- Mycoplasma salivarium
- Mycoplasma synoviae
- Spiroplasma citri
- Ureaplasma urealyticum
- Mycoplasma pirum
- Mesomycoplasma hyopneumonia
What is the Internal Amplification Control?
MycoDetect includes an Internal Amplification Control (IAC) in the detection mix. The IAC is amplified alongside the mycoplasma target and is detected using the HEX fluorescence channel.The internal control helps confirm that the qPCR reaction has performed as expected. Failure of the IAC to amplify in an otherwise negative sample may indicate an unsuccessful reaction or possible PCR inhibition and should be investigated before interpreting the sample as negative.
How does MycoDetect help prevent PCR carryover contamination?
MycoDetect contains MegaMix Platinum Probe qPCR Mastermix with dUTP and UNG. The dUTP/UNG system helps prevent previous PCR amplification products from generating false-positive results in subsequent assays by targeting uracil-containing carryover amplicons before amplification.
Instrument Compatibility
MycoDetect can be used with qPCR instruments capable of detecting FAM and HEX fluorescence.
Mycoplasma: FAM
Internal Amplification Control: HEX
*Users should confirm that their instrument supports these fluorescence channels before running the assay.
Workflow summary:
Sample → Reaction Setup → qPCR → FAM/HEX Detection → Result
- Results in <1 hour
- Probe-based qPCR detection
- Targets a highly conserved mycoplasma 16S-rDNA region
- Detects common Mollicutes associated with cell-culture contamination
- Internal Amplification Control included
- Positive control included
- Mycoplasma-specific detection through FAM
- Internal-control detection through HEX
- dUTP/UNG carryover-contamination control
- Contains all components required for qPCR
- No additional qPCR optimisation required
- Compatible with qPCR cyclers capable of detecting FAM and HEX
- 100-reaction kit
- Designed for routine laboratory mycoplasma screening.
- Routine screening of cell cultures for mycoplasma contamination
- Research laboratories maintaining mammalian cell cultures
- Laboratories requiring rapid mycoplasma screening
- Laboratories with FAM/HEX-compatible real-time PCR instruments
- Researchers wanting an internal amplification control in every reaction
- Laboratories looking to reduce PCR carryover contamination using dUTP/UNG
- Routine contamination monitoring before critical cell-based experiments
- Checking cell cultures where mycoplasma contamination is suspected
- Product name: MycoDetect – Mycoplasma qPCR Detection Kit
- Brand: Microzone
- SKU: MYCO-100
- Description probe-based qPCR detection of common mycoplasma contaminants in cell cultures, with built-in internal and positive controls and results in less than one hour.
- Availability: same day shipping from orders placed before noon.
- Price: £495
- Target: Mycoplasma 16S-rDNA
- Run time: <1 hour
- Detection method: Probe-based
- Mycoplasma channel: FAM
- Internal control channel: HEX
- Sample types: cell-culture supernatants
- Intended use: Research use only
What is the MycoDetect Mycoplasma qPCR Detection Kit?
Answer: MycoDetect is a probe-based qPCR kit designed for rapid detection of mycoplasma contamination in cell cultures. It targets a highly conserved 16S-rDNA region and includes an Internal Amplification Control and positive-control DNA.
What is mycoplasma contamination?
Answer: Mycoplasma contamination occurs when cell cultures become infected with small bacteria belonging to the Mollicutes group. Contamination can affect cellular metabolism, gene expression and growth, potentially compromising experimental results.
Why should cell cultures be tested for mycoplasma?
Answer: Mycoplasma contamination can remain unnoticed during routine cell culture while affecting cell behaviour and experimental reproducibility. Routine screening helps identify contaminated cultures before they compromise downstream research.
How does MycoDetect detect mycoplasma?
Answer: MycoDetect uses probe-based qPCR to amplify a conserved mycoplasma 16S-rDNA region. Mycoplasma-specific amplification is detected through the FAM fluorescence channel.
What region does MycoDetect target?
Answer: MycoDetect targets a highly conserved mycoplasma 16S-rDNA region, allowing detection of common Mollicutes associated with cell-culture contamination.
Which mycoplasma species can MycoDetect detect?
Answer: The detection spectrum includes M. arginini, M. fermentans, M. hominis, M. hyorhinis, M. orale, M. pneumoniae and A. laidlawii.
Can MycoDetect detect Mycoplasma hyorhinis?
Answer: Yes. Mycoplasma hyorhinis is specifically included in the detection spectrum listed for MycoDetect.
Can MycoDetect detect Mycoplasma orale?
Answer: Yes. MycoDetect is designed to detect M. orale, and the supplied positive control contains a synthetic DNA sequence derived from M. orale.
Can MycoDetect detect Acholeplasma laidlawii?
Answer: Yes. Acholeplasma laidlawii is specifically included among the Mollicutes detected by MycoDetect.
How quickly can MycoDetect detect mycoplasma?
Answer: MycoDetect is designed to provide results in less than one hour using the recommended qPCR protocol.
What is the Internal Amplification Control in MycoDetect?
Answer: The Internal Amplification Control, or IAC, is amplified alongside the mycoplasma assay and detected through the HEX channel. It provides an internal check that the qPCR reaction has performed correctly.
Does MycoDetect include a positive control?
Answer: Yes. MycoDetect includes a synthetic mycoplasma positive-control DNA containing a sequence from M. orale.
How does MycoDetect help prevent PCR carryover contamination?
Answer: The supplied MegaMix Platinum qPCR Mastermix contains dUTP and UNG, which help prevent previous PCR products from causing carryover-related false-positive results.
Which qPCR instruments are compatible with MycoDetect?
Answer: MycoDetect is compatible with qPCR cyclers capable of detecting FAM and HEX fluorescence. Users should confirm that both channels are supported by their instrument.
Which fluorescence channels does MycoDetect use?
Answer: Mycoplasma-specific amplification is detected through FAM, while the Internal Amplification Control is detected through HEX.
Does MycoDetect require qPCR optimisation?
Answer: The kit contains the components required for qPCR and is supplied with an established protocol, so no additional qPCR optimisation is required for the recommended assay conditions.
What equipment is required to use MycoDetect?
Answer: A real-time PCR instrument capable of detecting FAM and HEX fluorescence is required, together with normal PCR laboratory equipment such as suitable PCR tubes or plates and calibrated pipettes.
How many reactions are included in MycoDetect?
Answer: The MYCO-100 kit contains sufficient reagents for 100 qPCR reactions.
Is MycoDetect for diagnostic use?
Answer: No. The current Microzone documentation identifies MycoDetect as for research use only.
Why choose MycoDetect for mycoplasma detection?
Answer: Choose MycoDetect when you need a rapid probe-based qPCR test for routine mycoplasma screening with results in less than one hour, built-in internal and positive controls, broad FAM/HEX instrument compatibility and dUTP/UNG carryover-contamination control.
- Results in <1 hour
- Probe-based qPCR detection
- Targets a highly conserved mycoplasma 16S-rDNA region
- Detects common Mollicutes associated with cell-culture contamination
- Internal Amplification Control included
- Positive control included
- Mycoplasma-specific detection through FAM
- Internal-control detection through HEX
- dUTP/UNG carryover-contamination control
- Contains all components required for qPCR
- No additional qPCR optimisation required
- Compatible with qPCR cyclers capable of detecting FAM and HEX
- 100-reaction kit
- Designed for routine laboratory mycoplasma screening.
- Routine screening of cell cultures for mycoplasma contamination
- Research laboratories maintaining mammalian cell cultures
- Laboratories requiring rapid mycoplasma screening
- Laboratories with FAM/HEX-compatible real-time PCR instruments
- Researchers wanting an internal amplification control in every reaction
- Laboratories looking to reduce PCR carryover contamination using dUTP/UNG
- Routine contamination monitoring before critical cell-based experiments
- Checking cell cultures where mycoplasma contamination is suspected
- Product name: MycoDetect – Mycoplasma qPCR Detection Kit
- Brand: Microzone
- SKU: MYCO-100
- Description probe-based qPCR detection of common mycoplasma contaminants in cell cultures, with built-in internal and positive controls and results in less than one hour.
- Availability: same day shipping from orders placed before noon.
- Price: £495
- Target: Mycoplasma 16S-rDNA
- Run time: <1 hour
- Detection method: Probe-based
- Mycoplasma channel: FAM
- Internal control channel: HEX
- Sample types: cell-culture supernatants
- Intended use: Research use only
What is the MycoDetect Mycoplasma qPCR Detection Kit?
Answer: MycoDetect is a probe-based qPCR kit designed for rapid detection of mycoplasma contamination in cell cultures. It targets a highly conserved 16S-rDNA region and includes an Internal Amplification Control and positive-control DNA.
What is mycoplasma contamination?
Answer: Mycoplasma contamination occurs when cell cultures become infected with small bacteria belonging to the Mollicutes group. Contamination can affect cellular metabolism, gene expression and growth, potentially compromising experimental results.
Why should cell cultures be tested for mycoplasma?
Answer: Mycoplasma contamination can remain unnoticed during routine cell culture while affecting cell behaviour and experimental reproducibility. Routine screening helps identify contaminated cultures before they compromise downstream research.
How does MycoDetect detect mycoplasma?
Answer: MycoDetect uses probe-based qPCR to amplify a conserved mycoplasma 16S-rDNA region. Mycoplasma-specific amplification is detected through the FAM fluorescence channel.
What region does MycoDetect target?
Answer: MycoDetect targets a highly conserved mycoplasma 16S-rDNA region, allowing detection of common Mollicutes associated with cell-culture contamination.
Which mycoplasma species can MycoDetect detect?
Answer: The detection spectrum includes M. arginini, M. fermentans, M. hominis, M. hyorhinis, M. orale, M. pneumoniae and A. laidlawii.
Can MycoDetect detect Mycoplasma hyorhinis?
Answer: Yes. Mycoplasma hyorhinis is specifically included in the detection spectrum listed for MycoDetect.
Can MycoDetect detect Mycoplasma orale?
Answer: Yes. MycoDetect is designed to detect M. orale, and the supplied positive control contains a synthetic DNA sequence derived from M. orale.
Can MycoDetect detect Acholeplasma laidlawii?
Answer: Yes. Acholeplasma laidlawii is specifically included among the Mollicutes detected by MycoDetect.
How quickly can MycoDetect detect mycoplasma?
Answer: MycoDetect is designed to provide results in less than one hour using the recommended qPCR protocol.
What is the Internal Amplification Control in MycoDetect?
Answer: The Internal Amplification Control, or IAC, is amplified alongside the mycoplasma assay and detected through the HEX channel. It provides an internal check that the qPCR reaction has performed correctly.
Does MycoDetect include a positive control?
Answer: Yes. MycoDetect includes a synthetic mycoplasma positive-control DNA containing a sequence from M. orale.
How does MycoDetect help prevent PCR carryover contamination?
Answer: The supplied MegaMix Platinum qPCR Mastermix contains dUTP and UNG, which help prevent previous PCR products from causing carryover-related false-positive results.
Which qPCR instruments are compatible with MycoDetect?
Answer: MycoDetect is compatible with qPCR cyclers capable of detecting FAM and HEX fluorescence. Users should confirm that both channels are supported by their instrument.
Which fluorescence channels does MycoDetect use?
Answer: Mycoplasma-specific amplification is detected through FAM, while the Internal Amplification Control is detected through HEX.
Does MycoDetect require qPCR optimisation?
Answer: The kit contains the components required for qPCR and is supplied with an established protocol, so no additional qPCR optimisation is required for the recommended assay conditions.
What equipment is required to use MycoDetect?
Answer: A real-time PCR instrument capable of detecting FAM and HEX fluorescence is required, together with normal PCR laboratory equipment such as suitable PCR tubes or plates and calibrated pipettes.
How many reactions are included in MycoDetect?
Answer: The MYCO-100 kit contains sufficient reagents for 100 qPCR reactions.
Is MycoDetect for diagnostic use?
Answer: No. The current Microzone documentation identifies MycoDetect as for research use only.
Why choose MycoDetect for mycoplasma detection?
Answer: Choose MycoDetect when you need a rapid probe-based qPCR test for routine mycoplasma screening with results in less than one hour, built-in internal and positive controls, broad FAM/HEX instrument compatibility and dUTP/UNG carryover-contamination control.
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