MegaMix Mastermix

The first few cycles of PCR are always the most important to successful amplification. Our mastermixes are never short of enough polymerase to kick start and then powerfully continue your amplification. MegaMix solutions are designed to cope with whatever is thrown at them. We incorporate two different Taq polymerases. The two Taqs complement each other and with the addition of Microzone’s own proprietary engineered PCR buffers, our mastermixes provide powerful, broad template amplification.

  • As close as you’ll get to a one mix fits all.
  • Exceptionally stable and can be freeze thawed on multiple occasions.
  • High success rates up to 6kb.
  • High yields under standard conditions.
  • Provides effective and efficient amplification for GC and AT rich templates.
  • Strong MgCl2 to support strong amplification.
  • Available as MegaMix (1x Conc) and MegaMix Double (2x Conc)


  • More confidence in amplification and PCR test.
  • Mastermix format is ready to use or as a 2x concentration.
  • Both single and double concentrations. Get the right mix for your tests quickly and easily.
  • Easy set up and PCR optimisation.
  • Broad range of templates and conditions.
  • Routine end point PCR.
  • Fast PCR.
  • Genotyping.
  • Amplification of GC and AT rich templates.
  • Up to 6kb.
  • Methylated DNA.
  • TA Cloning

Cycling Profile (guide only)

  • Initial denaturation step: 95⁰C for 3 mins
  • Then Cycle 25-30 times:
  • Step 1: 95⁰C for 30 secs
  • Step 2: Optimal annealing temp. of primers for 30 to 60 secs
  • Step 3: 72⁰C for 45 to 60 secs
  • Cool to room temperature
  • Thaw reagents prior to use. Mix well prior to use.
  • GC and complex templates may require higher denaturation temperatures
  • Two step cycling protocols can increase speed. Following annealing just ramp the temperature back to denaturation.
  • If using new primers or changing your master mix it will be necessary to perform a new annealing temperature gradient. We suggest 1⁰C intervals and to start at 5⁰C below the calculated Tm of the primers and go to 5⁰C above the primer Tm.
  • Primers design and quality have a large impact on the reaction. Taking time to design your primers using good techniques or software will benefit down the line. We do offer a technical note on primer design. Contact us to receive a copy.
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Since 1998, Microzone has been at the forefront of empowering scientists in their DNA & RNA research. Our legacy is built on a comprehensive suite of products tailored for DNA/RNA extraction, purification, amplification, post-PCR clean-up, and sequencing. From PCR, qPCR, RT-qPCR, and HRM tools to advanced solutions like magnetic bead extraction and direct-to-PCR extraction buffers, we've consistently delivered precision and reliability. Choose Microzone, and harness two decades of expertise dedicated to advancing molecular biology.