2MMB

MegaMix Blue PCR Mastermix

£17.00£1,210.00

Description

MegaMix Blue End Point PCR Mastermix:

One of Microzone’s first MegaMixes. Over the years this MegaMix has stood tall. It’s still a firm favourite with many customers saying it’s their go to end point PCR mastermix.

From the start we knew that the first few cycles of PCR are always the most important to successful amplification. Therefore, our mastermixes are never short of enough polymerase to kick start and then powerfully continue your amplification. MegaMix Blue (and MegaMix) are designed to cope with whatever is thrown at them. We incorporate two different Taq polymerases. The two Taqs complement each other and offer customers the capability to cover a large range of templates with just one mastermix.  The addition of Microzone’s own proprietary engineered PCR buffer ensures that our mastermixes provide powerful, broad template, amplification.

Looking for a non-hot start PCR mastermix? You can’t go far wrong with our tried and fully tested over many years MegaMix Blue or MegaMix PCR mastermixes.

MegaMix – by Microzone

Microzone’s MegaMix range of PCR mastermixes covers a broad range of a scientist’s requirements. We offer a MegaMix for most occasions including:

  • Routine End Point PCR
  • Hot Start End Point PCR
  • qPCR
  • RT-qPCR
  • High Fidelity (HiFi) PCR
  • Inhibitor Resistant PCR
  • High Resolution Melting
  • RT-PCR

Associated products include – Taq’s with buffer; dNTP mixes; Molecular Grade Water…….

Additional information

Quantity

Megamix Blue 1mL, Megamix Blue 5 X 1 mL, Megamix Blue 25 X 1 mL, Megamix Blue 100 X 1 mL, Megamix Blue Double 1mL, Megamix Blue Double 5 X 0.5 mL, Megamix Blue Double 25 X 0.5 mL, Megamix Blue Double 100 X 0.5 mL

MegaMix Blue End Point PCR Mastermix:

One of Microzone's first MegaMixes. Over the years this MegaMix has stood tall. It's still a firm favourite with many customers saying it's their go to end point PCR mastermix.

From the start we knew that the first few cycles of PCR are always the most important to successful amplification. Therefore, our mastermixes are never short of enough polymerase to kick start and then powerfully continue your amplification. MegaMix Blue (and MegaMix) are designed to cope with whatever is thrown at them. We incorporate two different Taq polymerases. The two Taqs complement each other and offer customers the capability to cover a large range of templates with just one mastermix.  The addition of Microzone’s own proprietary engineered PCR buffer ensures that our mastermixes provide powerful, broad template, amplification.

Looking for a non-hot start PCR mastermix? You can't go far wrong with our tried and fully tested over many years MegaMix Blue or MegaMix PCR mastermixes.

MegaMix - by Microzone

Microzone's MegaMix range of PCR mastermixes covers a broad range of a scientist's requirements. We offer a MegaMix for most occasions including:

  • Routine End Point PCR
  • Hot Start End Point PCR
  • qPCR
  • RT-qPCR
  • High Fidelity (HiFi) PCR
  • Inhibitor Resistant PCR
  • High Resolution Melting
  • RT-PCR

Associated products include - Taq's with buffer; dNTP mixes; Molecular Grade Water.......

  • Specially formulated Taq polymerase blend in optimised buffer for efficient PCR
    amplification.
  • Inert blue loading dye for direct gel loading.
  • Available as MegaMix Blue (1x Conc) and MegaMix Blue Double (2x Conc)
  • More confidence in amplification and PCR test.
  • Mastermix format is ready to use or as a 2x concentration.
  • Both single and double concentrations. Get the right mix for your tests quickly and easily.
  • Easy set up and PCR optimisation.
  • Broad range of templates and conditions.
  • Routine end point PCR.
  • Fast PCR.
  • Genotyping.
  • Amplification of GC and AT rich templates.
  • Up to 6kb.
  • Methylated DNA.
  • TA Cloning

Cycling Profile (guide only)

  • Initial denaturation step: 95⁰C for 3 mins
  • Then Cycle 25-30 times:
  • Step 1: 95⁰C for 30 secs
  • Step 2: Optimal annealing temp. of primers for 30 to 60 secs
  • Step 3: 72⁰C for 45 to 60 secs
  • Cool to room temperature
  • Thaw reagents prior to use. Mix well prior to use.
  • GC and complex templates may require higher denaturation temperatures
  • Two step cycling protocols can increase speed. Following annealing just ramp the temperature back to denaturation.
  • If using new primers or changing your master mix it will be necessary to perform a new annealing temperature gradient. We suggest 1⁰C intervals and to start at 5⁰C below the calculated Tm of the primers and go to 5⁰C above the primer Tm.
  • Primers design and quality have a large impact on the reaction. Taking time to design your primers using good techniques or software will benefit down the line. We do offer a technical note on primer design. Contact us to receive a copy.
MegaMix Blue Protocol MegaMix Blue + MegaMix Blue Double Material Safety Data Sheet Megamix Blue Double Protocol

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