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MegaMix HRM Mastermix
£56.00 – £500.00
Description
MegaMix HRM – High Resolution Melting PCR Mastermix
MegaMix HRM contains all the components needed to perform HRM-PCR swiftly and reliably. The 2X mix contains chemically modified Hot Start Taq DNA polymerase and microGREEN intercalating dye in enhancing buffer optimised for HRM analysis. microGREEN is a third generation, saturating, intercalating fluorescent dye that binds to double stranded DNA without inhibiting PCR; making MegaMix HRM the perfect choice for HRM.
The Hot Start Taq polymerase is chemically inactivated until heating to 95°C, providing excellent sensitivity and specificity; eliminating the formation of non-specific amplification and primer-dimers.
This mastermix performs excellently when amplifying from GC rich templates, proving reproducible and reliable results with little or no optimisation.
MegaMix – by Microzone
Microzone’s MegaMix range of PCR mastermixes covers a broad range of a scientist’s requirements. We offer a MegaMix for most occasions including:
- Routine End Point PCR
- Hot Start End Point PCR
- qPCR
- RT-qPCR
- High Fidelity (HiFi) PCR
- Inhibitor Resistant PCR
- High Resolution Melting
- RT-PCR
Associated products include – Taq’s with buffer; dNTP mixes; Molecular Grade Water…….
Additional information
| Quantity | 1mL, 5 x 1mL, 10 x 1mL |
|---|
MegaMix HRM - High Resolution Melting PCR Mastermix
MegaMix HRM contains all the components needed to perform HRM-PCR swiftly and reliably. The 2X mix contains chemically modified Hot Start Taq DNA polymerase and microGREEN intercalating dye in enhancing buffer optimised for HRM analysis. microGREEN is a third generation, saturating, intercalating fluorescent dye that binds to double stranded DNA without inhibiting PCR; making MegaMix HRM the perfect choice for HRM.
The Hot Start Taq polymerase is chemically inactivated until heating to 95°C, providing excellent sensitivity and specificity; eliminating the formation of non-specific amplification and primer-dimers.
This mastermix performs excellently when amplifying from GC rich templates, proving reproducible and reliable results with little or no optimisation.
MegaMix - by Microzone
Microzone's MegaMix range of PCR mastermixes covers a broad range of a scientist's requirements. We offer a MegaMix for most occasions including:
- Routine End Point PCR
- Hot Start End Point PCR
- qPCR
- RT-qPCR
- High Fidelity (HiFi) PCR
- Inhibitor Resistant PCR
- High Resolution Melting
- RT-PCR
Associated products include - Taq's with buffer; dNTP mixes; Molecular Grade Water.......
- Incorporates Microzone’s proprietary third generation, non-inhibitory intercalating
dye, microGREEN, at a saturating concentration ideal for the most sensitive of HRM
applications.
- Accurately genotype large number of samples in a short time, with a high level of
accuracy with MegaMix Gold HRM. - More cost effective genotyping compared to sequencing and Taqman SNP typing.
High Resolution Melt (HRM) analysis:
- Single Nucleotide Polymorphism (SNP) genotyping
- Methylation analysis
- Mutation scanning
- Detection of sequence variations
Available in the following formats:
1mL
5 x 1mL
10 x 1mL
Fast Delivery
Order before 12:00 GMT, dispatched today.
Dedicated Support
Our team is always on hand to provide assistance.
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Join over 2000 satisfied Microzone customers.
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At Microzone, we recognise the unique demands of every laboratory. Whether you're ramping up experiments or seeking specialised formulations, our dedicated team is on standby. Contact us today and benefit from personalised attention that ensures your order is precisely tailored.
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