MegaMix HRM Mastermix

£90.00£750.00

Description

MegaMix HRM simplifies HRM-PCR with its 2X mix featuring chemically modified Hot Start Taq polymerase and microGREEN dye in an optimized buffer for HRM analysis. The polymerase is inactive until 95°C, ensuring high sensitivity and specificity, preventing non-specific amplification. Ideal for GC-rich templates, it delivers reproducible results with minimal optimization.

Additional information

Quantity

1mL, 5 x 1mL, 10 x 1mL

MegaMix HRM simplifies HRM-PCR with its 2X mix featuring chemically modified Hot Start Taq polymerase and microGREEN dye in an optimized buffer for HRM analysis. The polymerase is inactive until 95°C, ensuring high sensitivity and specificity, preventing non-specific amplification. Ideal for GC-rich templates, it delivers reproducible results with minimal optimization.

  • Save Time and Money—quickly and efficiently identify sequence variations.
  • Specificity—Hot Start Taq DNA Polymerase in optimised buffer eliminates non-specific amplification and the formation of primer dimers.
  • Sensitivity—detect class 4 SNP.
  • Versatile— compatible with standard and fast cycling conditions, GC/AT rich templates.
  • Reproducibility and convenience—ready to use 2X format.
  • Third generation intercalating dye— no inhibition of PCR, even at high concen-trations, suitable for High Resolution Melting.

High Resolution Melt (HRM) analysis:

  • Single Nucleotide Polymorphism (SNP) genotyping
  • Methylation analysis
  • Mutation scanning
  • Detection of sequence variations

Available in the following formats:

1mL

5 x 1mL

10 x 1mL

Protocol

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David, UK

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