2MME-RTU

Dye based One Step RT-qPCR Mastermix

£101.00£825.00

Description

Everything you need for efficient multiplex RT-qPCR in a single reaction, swiftly and reliably. The 2X mix contains chemically modified Hot Start Taq DNA polymerase, intercalating dye, dUTP and UNG in enhancing buffer optimised for amplifying low copy RNA targets. The third generation intercalating fluorescent dye binds to double stranded DNA, making MegaMix Emerald the perfect choice for qPCR, Melt Curve Analysis and High Resolution Melting (HRM).

Additional information

Quantity

100 Reactions, 200 Reactions, 1000 Reactions

Everything you need for efficient multiplex RT-qPCR in a single reaction, swiftly and reliably. The 2X mix contains chemically modified Hot Start Taq DNA polymerase, intercalating dye, dUTP and UNG in enhancing buffer optimised for amplifying low copy RNA targets. The third generation intercalating fluorescent dye binds to double stranded DNA, making MegaMix Emerald the perfect choice for qPCR, Melt Curve Analysis and High Resolution Melting (HRM).

  • Incorporates Microzone’s proprietary third generation, none-inhibitory intercalating
    dye, microGREEN, for increased fluorescence and unmatched sensitivity.
  • Confidence inspiring, incorporation of thermolabile UNG and dUTP’s prevent
    carryover contamination and allow for use in downstream applications.
  • Compatible with any qPCR instrument with a FAM/SYBR channel.
  • One-Step format slashes time and effort required to get reliable results.
  • qPCR along with reverse transcription all in one tube
  • Simple set up
  • Can be used with both standard and fast cycling.
  • Perfect for qPCR as it doesn’t interfere with PCR. At saturation levels it is also excellent for HRM.

Available in 200 & 1000 reactions.

Protocol

Order before 12:00 GMT, dispatched today.

Our team is always on hand to provide assistance.

Join over 2000 satisfied Microzone customers.

David, UK

Custom Orders Made Easy

At Microzone, we recognise the unique demands of every laboratory. Whether you're ramping up experiments or seeking specialised formulations, our dedicated team is on standby. Contact us today and benefit from personalised attention that ensures your order is precisely tailored.

Contact Sales Team