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2MMEU
MegaMix Emerald qPCR Mastermix (Dye Based)
Price range: £45.00 through £450.00
Description
MegaMix Emerald qPCR Mastermix all the components needed to perform dye-based qPCR swiftly and reliably. The 2X mix contains chemically modified Hot Start Taq DNA polymerase, intercalating dye (microGREEN), dUTP/dNTP and UNG (if carryover contamination control is required) in enhancing Microzone’s proprietary PCR buffer optimised for amplifying low copy DNA targets.
We have incorporated microGREEN Dye (a third generation intercalating highly fluorescent dye that binds to double stranded DNA) into MegaMix Emerald. This dye mix combinations makes MegaMix Emerald the perfect choice for qPCR and Melt Curve Analysis.
The Hot Start Taq polymerase is chemically inactivated until heating to 95°C, providing excellent sensitivity and specificity; eliminating the formation of non-specific amplification and primer-dimers.
Available with optional UNG and dUTP’s to eliminate carryover contamination, the thermolabile UNG is active at room temperature before being completely and irreversibly inactivated when heated to 95°C. This means your PCR product is suitable for downstream processing unlike traditional UNG.
MegaMix Emerald using MicroGreen dye ensures high sensitivity and an earlier Cq than competitor mixes. When combined with Microzone’s SwiftScript cDNA synthesis product, you achieve the perfect combination for RT-qPCR too.
Ultra Fast PCR – MegaMix Emerald is capable to powering your PCR to new heights. With 5” denaturation 1” annealing a complete PCR can be completed in just over 30 minutes. You can download our full application paper here!
MegaMix Emerald is stable for different periods at -20°C, 4°C and RT and at 40°C.
This product is available premixed Low and High concentrations of ROX reference dye. Low ROX mix is compatible with instruments that do not require ROX reference dye. Please contact Microzone for recommendations specific to your qPCR instrument.
Additional information
| Quantity | 100 Reactions, 200 Reactions, 1000 Reactions |
|---|---|
| ROX | Lo ROX (Compatible with Lo and No ROX instruments), Hi-ROX |
| UNG | without UNG, with UNG |
MegaMix Emerald qPCR Mastermix all the components needed to perform dye-based qPCR swiftly and reliably. The 2X mix contains chemically modified Hot Start Taq DNA polymerase, intercalating dye (microGREEN), dUTP/dNTP and UNG (if carryover contamination control is required) in enhancing Microzone's proprietary PCR buffer optimised for amplifying low copy DNA targets.
We have incorporated microGREEN Dye (a third generation intercalating highly fluorescent dye that binds to double stranded DNA) into MegaMix Emerald. This dye mix combinations makes MegaMix Emerald the perfect choice for qPCR and Melt Curve Analysis.
The Hot Start Taq polymerase is chemically inactivated until heating to 95°C, providing excellent sensitivity and specificity; eliminating the formation of non-specific amplification and primer-dimers.
Available with optional UNG and dUTP’s to eliminate carryover contamination, the thermolabile UNG is active at room temperature before being completely and irreversibly inactivated when heated to 95°C. This means your PCR product is suitable for downstream processing unlike traditional UNG.
MegaMix Emerald using MicroGreen dye ensures high sensitivity and an earlier Cq than competitor mixes. When combined with Microzone's SwiftScript cDNA synthesis product, you achieve the perfect combination for RT-qPCR too.
Ultra Fast PCR - MegaMix Emerald is capable to powering your PCR to new heights. With 5'' denaturation 1'' annealing a complete PCR can be completed in just over 30 minutes. You can download our full application paper here!
MegaMix Emerald is stable for different periods at -20°C, 4°C and RT and at 40°C.
This product is available premixed Low and High concentrations of ROX reference dye. Low ROX mix is compatible with instruments that do not require ROX reference dye. Please contact Microzone for recommendations specific to your qPCR instrument.
MegaMix Emerald Demonstrates Excellent Reproducibility and Linearity When Amplifying from Saliva gDNA. MegaMix Emerald qPCR amplification of hp53 from serially diluted saliva gDNA extracted using Microzone Blood Tissue Saliva Extraction Kit.
- Incorporates Microzone’s proprietary third generation, non-inhibitory intercalating dye, microGREEN, for increased fluorescence and unmatched sensitivity.
- Eliminates carry over contamination—incorporation of thermolabile UNG and dUTP prevent amplicon contamination from previous runs without degrading generated amplicons.
- Compatible with any qPCR instrument with a FAM/SYBR channel.
- Specificity—Hot Start Taq DNA Polymerase in optimized buffer eliminates non-specific amplification and the formation of primer dimers.
- Sensitivity—Single copy detection.
- Versatile— compatible with standard and fast cycling conditions, GC/AT rich templates.
- Reproducibility and convenience—ready to use 2X format.
- Inhibitor Resistance—suitable for direct to PCR with products such as microLYSIS-Plus and microLYSIS-RNA.
- Third generation intercalating dye— no inhibition of PCR, even at high concentrations, suitable for High Resolution Melting.
- Dye based qPCR
- Use with gDNA, Viral DNA, cDNA….
- Use for quantification, gene expression, SNP detection using High Resolution Melting (HRM).
MegaMix Emerald qPCR with UNG (orange) exhibits earlier Cq values and superior sensitivity vs competitor A (black), when amplifying the RNase P gene from a 6X, 1 in 10 serial dilution of 1 µg human DNA. BMS MIC.
- Incorporates Microzone’s proprietary third generation, non-inhibitory intercalating dye, microGREEN, for increased fluorescence and unmatched sensitivity.
- Eliminates carry over contamination—incorporation of thermolabile UNG and dUTP prevent amplicon contamination from previous runs without degrading generated amplicons.
- Compatible with any qPCR instrument with a FAM/SYBR channel.
- Specificity—Hot Start Taq DNA Polymerase in optimized buffer eliminates non-specific amplification and the formation of primer dimers.
- Sensitivity—Single copy detection.
- Versatile— compatible with standard and fast cycling conditions, GC/AT rich templates.
- Reproducibility and convenience—ready to use 2X format.
- Inhibitor Resistance—suitable for direct to PCR with products such as microLYSIS-Plus and microLYSIS-RNA.
- Third generation intercalating dye— no inhibition of PCR, even at high concentrations, suitable for High Resolution Melting.
- Dye based qPCR
- Use with gDNA, Viral DNA, cDNA….
- Use for quantification, gene expression, SNP detection using High Resolution Melting (HRM).
MegaMix Emerald qPCR with UNG (orange) exhibits earlier Cq values and superior sensitivity vs competitor A (black), when amplifying the RNase P gene from a 6X, 1 in 10 serial dilution of 1 µg human DNA. BMS MIC.
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