Search
megamix-emerald -qPCR-dye-based

MegaMix Emerald qPCR Mastermix (Dye Based)

Everything you need for efficient qPCR in a single reaction, swiftly and reliably. The 2X mix contains chemically modified Hot Start Taq DNA polymerase, intercalating dye (microGREEN), dUTP and UNG in enhancing buffer, optimised for amplifying low-copy DNA targets. The third-generation intercalating fluorescent dye (microGREEN), binds to double-stranded DNA, making MegaMix Emerald the perfect choice for qPCR, Melt Curve Analysis and is ideal for use with MegaMix HRM (High Resolution Melting).

  • High success rates up to 6kb.
  • High yields under standard conditions.
  • Provides effective and efficient amplification for GC and AT rich templates.
  • Strong MgCl2 to support strong amplification.

£56.00£450.00

The Hot Start Taq polymerase is chemically inactivated until heating to 95°C, providing excellent sensitivity and specificity; eliminating the formation of non-specific amplification and primer-dimers. The presence of UNG and dUTP’s eliminates carryover contamination, the thermolabile UNG is active at room temperature before being completely and irreversibly inactivated when heated to 95°C, this means your PCR product is suitable for downstream processing.

  • Can be used with standard and fast cycling and amplification.
  • Works well with dye based assays.
  • PCR water supplied with kit
  • Comes with dUTP and UNG
  • Dye based qPCR
  • Use with gDNA, Viral DNA, cDNA….
  • Use for quantification, gene expression, SNP detection using High Resolution Melting (HRM).

MegaMix Emerald qPCR with UNG (orange) exhibits earlier Cq values and superior sensitivity vs competitor A (black), when amplifying the RNase P gene from a 6X, 1 in 10 serial dilution of 1 µg human DNA. BMS MIC.

MegaMix Emerald qPCR

Fast Delivery

Order before 12:00 GMT, dispatched today.

Dedicated Support

Our customer service team is always on hand to provide assistance.

Satisfaction Guarantee

Choose Microzone and join over 2000 satisfied customers.

Product Downloads

Download essential technical information for this product.

David

UK

Custom Orders Made Easy

At Microzone, we recognise the unique demands of every laboratory. Whether you're ramping up experiments or seeking specialised formulations, our dedicated team is on standby. Contact us today and benefit from personalised attention that ensures your order is precisely tailored

Explore Our Catalogue

Why Microzone?

Since 1998, Microzone has been at the forefront of empowering scientists in their DNA & RNA research. Our legacy is built on a comprehensive suite of products tailored for DNA/RNA extraction, purification, amplification, post-PCR clean-up, and sequencing. From PCR, qPCR, RT-qPCR, and HRM tools to advanced solutions like magnetic bead extraction and direct-to-PCR extraction buffers, we've consistently delivered precision and reliability. Choose Microzone, and harness two decades of expertise dedicated to advancing molecular biology.