MegaMix Gold RT-PCR Mastermix

The MegaMix Gold One-step RT-PCR kit contains all the components needed to perform RT-PCR swiftly and reliably. The kit contains all the components needed to perform RT-PCR swiftly and reliably. The kit ensures a highly specific and sensitive amplification, removing background and primer dimer formation. MegaMix Gold boasts excellent accuracy and produces A-tailed products suitable for ligating into TA cloning vectors.

  • Hot Start polymerase in Microzone’s proprietary buffer gives unrivalled confidence in RT-PCR amplifications.
  • 2X concentrated format.
  • Broad range of templates and conditions.
  • Extremely stable—can be freeze-thawed many times.
  • Easy setup and RT-PCR optimisation.


  • One step RT-PCR in one reaction
  • Hot start polymerase reduces non-specific amplification
  • Extremely processive reverse transcriptase
  • Hot Start RT-PCR up to 6 kb
  • Amplification of any RNA template (mRNA, total RNA, viral RNA), low copy number genes
  • RT-qPCR (probe or dye based)
  • Endpoint RT-PCR
  • Pathogen Detection
  • Gene expression analysis.
  • Amplification of GC and AT rich templates

Available in the following formats:

200 reactions

1000 reactions

Fast Delivery

Order before 12:00 GMT, dispatched today.

Dedicated Support

Our customer service team is always on hand to provide assistance.

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Custom Orders Made Easy

At Microzone, we recognise the unique demands of every laboratory. Whether you're ramping up experiments or seeking specialised formulations, our dedicated team is on standby. Contact us today and benefit from personalised attention that ensures your order is precisely tailored

Why Microzone?

Since 1998, Microzone has been at the forefront of empowering scientists in their DNA & RNA research. Our legacy is built on a comprehensive suite of products tailored for DNA/RNA extraction, purification, amplification, post-PCR clean-up, and sequencing. From PCR, qPCR, RT-qPCR, and HRM tools to advanced solutions like magnetic bead extraction and direct-to-PCR extraction buffers, we've consistently delivered precision and reliability. Choose Microzone, and harness two decades of expertise dedicated to advancing molecular biology.