MegaMix Platinum qPCR Mastermix (Probe Based)
Price range: £45.00 through £515.00
Description
MegaMix Platinum Probe based qPCR Mastermix contains all the components needed to perform multiplex qPCR swiftly and reliably. The 2X mix contains chemically modified Hot Start Taq DNA polymerase, dUTP/dNTP and UNG (if carryover contamination control is required) in Microzone’s proprietary enhancing buffer optimised for amplifying low copy DNA targets.
Available with optional UNG and dUTP’s to eliminate carryover contamination, the thermolabile UNG is active at room temperature before being completely and irreversibly inactivated when heated to 95°C. This means your PCR product is suitable for downstream processing unlike traditional UNG.
This mix has been optimized for multiplex assays utilising dual labelled hydrolysis probes such as TaqMan and Scorpion®.
MegaMix Platinum is compatible with both standard and fast cycling conditions.
An excellent mix for anyone looking to create probe-based qPCR based kits.
High Temperature Stability – MegaMix Platinum was stored at 40°C over a period of 34 days. The product demonstrated the same activity using a triplex assay using TaqMan probes on day 34 as it did on day 1. Data shown in carousel. This supports the use of MegaMixPlatinum in hot environments where cold storage may be difficult to attain.
This product is also available premixed Low and High concentrations of ROX reference dye. Please contact Microzone for recommendations specific to your qPCR instrument.
MegaMix – by Microzone
Microzone’s MegaMix range of PCR mastermixes covers a broad range of a scientist’s requirements. We offer a MegaMix for most occasions including:
Associated products include – Taq’s with buffer; dNTP mixes; Molecular Grade Water…
Additional information
| Quantity | 100 Reactions, 200 Reactions, 1000 Reactions |
|---|---|
| ROX | No-ROX, Lo-ROX, Hi-ROX |
| UNG | without UNG, with UNG |
MegaMix Platinum Probe based qPCR Mastermix contains all the components needed to perform multiplex qPCR swiftly and reliably. The 2X mix contains chemically modified Hot Start Taq DNA polymerase, dUTP/dNTP and UNG (if carryover contamination control is required) in Microzone’s proprietary enhancing buffer optimised for amplifying low copy DNA targets.
Available with optional UNG and dUTP’s to eliminate carryover contamination, the thermolabile UNG is active at room temperature before being completely and irreversibly inactivated when heated to 95°C. This means your PCR product is suitable for downstream processing unlike traditional UNG.
This mix has been optimized for multiplex assays utilising dual labelled hydrolysis probes such as TaqMan and Scorpion®.
MegaMix Platinum is compatible with both standard and fast cycling conditions.
An excellent mix for anyone looking to create probe-based qPCR based kits.
High Temperature Stability - MegaMix Platinum was stored at 40°C over a period of 34 days. The product demonstrated the same activity using a triplex assay using TaqMan probes on day 34 as it did on day 1. Data shown in carousel. This supports the use of MegaMixPlatinum in hot environments where cold storage may be difficult to attain.
This product is also available premixed Low and High concentrations of ROX reference dye. Please contact Microzone for recommendations specific to your qPCR instrument.
MegaMix - by Microzone
Microzone's MegaMix range of PCR mastermixes covers a broad range of a scientist's requirements. We offer a MegaMix for most occasions including:
Associated products include - Taq's with buffer; dNTP mixes; Molecular Grade Water...
Standard Curve of MegaMix Platinum
qPCR_MMP_MRSA_MecC_Amplification Curve_1x10’11
MegaMix Platinum stored at 40ËšC in a controlled manner. Test were performed using a triplex assay over a period of 34 days. As can be seen at day 34 the results we comparable to day 1. Performance of MegaMix Platinum remained solid throughout the period tested. Testing was stopped at day 34.
- Robust linear amplification of multiple DNA targets for probe based detection.
- Confidence inspiring, incorporation of thermolabile UNG and dUTP’s prevent
carryover contamination and allow for use in downstream applications.
- Designed for use with probe based assays using TaqMan, Scorpions, Molecular Beacons etc.
- Detection and quantification of gDNA, cDNA, Viral DNA etc.
- Excellent for multiplex assays.
- Robust linear amplification of multiple DNA targets for probe based detection.
- Confidence inspiring, incorporation of thermolabile UNG and dUTP’s prevent
carryover contamination and allow for use in downstream applications.
- Designed for use with probe based assays using TaqMan, Scorpions, Molecular Beacons etc.
- Detection and quantification of gDNA, cDNA, Viral DNA etc.
- Excellent for multiplex assays.
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