MAGneat Bacteria Extraction

MAGneat gDNA Extraction Kit

Now optimised for Bacteria!

Kit contents and storage:

Product Description

Extracting bacterial DNA presents several challenges due to the diverse and complex nature of bacterial cell walls, contamination risks, and the need for high-quality DNA for downstream applications. Microzone’s MAGneat gDNA extraction kit addresses these challenges by providing efficient lysis, high DNA purity, and streamlined workflows, making bacterial DNA extraction faster, more reliable and efficient.

Protocol

Bacterial DNA extraction starts by centrifuging sample to pellet cells. Lysis is achieved by addition of Lysis Buffer and Proteinase K mastermix, followed by homogenisation using bead-beating technology. After centrifugation, DNA binds to MAGneat magnetic beads in the presence of the Binding Buffer, allowing efficient DNA recovery. Wash steps remove impurities and contaminants before elution with DNA elution buffer. Following a final magnetic separation, the purified DNA is then collected for downstream applications.

Figure 1. Microzone’s MAGneat magnetic separation devices work excellently with the MAGneat gDNA Extraction kit. Utilising neodymium magnets to ensure rapid and defined aggregation.

For detailed step-by-step instructions, download the MAGneat gDNA extraction kit’s Instructions For Use guide at microzone.co.uk

Key Features

•High Quality – MAGneat magnetic bead technology purifies high-molecular weight DNA.

Figure 2. High-molecular weight genomic DNA from both Gram-Positive and Gram-Negative bacteria extracted with MAGneat kit. 5 µL of the eluted DNA was loaded to 1% agarose gel. M = 1 kb DNA ladder.

•High Yield and Purity – Microzone’s proprietary buffers ensure the removal of contaminants and promote a high yield recovery.

Figure 3. DNA yield comparison from a range of bacteria species extracted using the MAGneat gDNA Extraction Kit and a comparator N. DNA concentration was measured using the Qubit 4 Fluorometer.

•Fast & Automatable – Magnetic bead technology is ideal for automation.

Figure 4. MAGneat gDNA Extraction Kit automates seamlessly with Microzone’s HeiDi-NA extraction system. Providing unrivaled performance and reproducibility.