High Resolution Melt Analysis: The Science Powering Cutting-Edge Research

High resolution melt analysis (HRM) transformed the landscape of genetic research, offering a robust, simple method for SNP genotyping, methylation analysis, mutation scanning, and sequence variation detection. By harnessing the precision of HRM, scientists can achieve exceptional, cost effective accuracy in their genetic studies.

MegaMix HRM Mastermix from Microzone is the ultimate solution for HRM analysis applications.

This mastermix is formulated to ensure optimal performance, providing consistent and reliable results. What sets MegaMix HRM apart is its proprietary dye, specifically designed to enhance the sensitivity and specificity of HRM assay applications. This unique dye, exclusive to Microzone, ensures excellent detection of genetic variations through enhanced fluorescence, making it a perfect tool for researchers seeking speed and reliability in their work.

By combining the advanced capabilities of high resolution melt analysis with the exceptional quality of MegaMix HRM Mastermix, Microzone delivers a comprehensive solution that empowers scientists in their work…

Download the Primer Design Document

What is High Resolution Melting (HRM)?

High Resolution Melt (HRM) analysis is a powerful technique used in molecular biology for the detection of variations in DNA sequences, such as SNP genotyping, methylation analysis, and mutation scanning. This method’s sensitivity to changes in melting temperature enables precise differentiation between even the smallest sequence variations, making it essential for accurate genotyping. At Microzone, we have extensive experience in HRM technology, having developed and refined our MegaMix HRM Mastermix entirely in-house in the UK. Our expertise ensures that each product delivers the highest quality and performance in every application.

**Why Choose MegaMix HRM Mastermix for Your Genotyping Needs?**

Looking for the best HRM reagent for mutation detection and genotyping in molecular biology? MegaMix HRM Mastermix from Microzone offers accuracy and convenience.

Unlock the full potential of your research with MegaMix HRM Mastermix. Engineered for efficiency and reliability, our mastermix ensures high sensitivity and specificity, making it an essential tool for any molecular biologist.

Key Features

High Sensitivity and Specificity: Hot Start Taq polymerase and optimized buffer prevent non-specific amplification and primer-dimers.
Advanced Dye Technology: Third-generation MicroGREEN dye ensures no PCR inhibition, even at high concentrations, dsDNA saturation for perfect high-resolution melting methods.
Versatile Applications: Compatible with both standard and fast cycling conditions, and suitable for GC/AT rich templates.
Consistent and Reliable: Achieve reproducible results with minimal optimization required.
User-Friendly: Ready-to-use 2X format simplifies your workflow, ideal for busy labs.
Cost-Effective: Save time and resources by quickly identifying sequence variations.

Benefits

Save Time and Money: Quickly and efficiently identify sequence variations crucial to genetic research.
Sensitive Detection: Detect class IV SNPs with high precision, enhancing the quality of your findings.
Convenient and Ready-to-Use: Simplify your workflow with the 2X ready-to-use format, perfect for busy microbiology labs.

How Does Microzone’s HRM Mastermix Compare To Competitors?

Real World Performance

Our research team has validated MegaMix HRM Mastermix across various genes and SNPs using high resolution melt analysis and fluorescence, taking into account the melting temperature to ensure precision.

**Download the MegaMix HRM Mastermix Primer Design Document**

Gain access to our Primer Design Document to see how MegaMix HRM Mastermix can transform your research.

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All MegaMix products are researched, designed, and manufactured in-house in the United Kingdom, ensuring the highest quality and reliability for your laboratory needs.

We Help Molecular Biologists Excel

With over 25 years of expertise, Microzone, founded by Dr. Hedda Steingrimsdottir, has become a leader in HRM technology. Our deep experience in developing and refining HRM products is reflected in the superior performance of our MegaMix HRM mastermix. Manufactured in our UK-based facility, we are committed to providing scientists with innovative and cost-effective tools designed to enhance genetic research and analysis.

If you’d like to find our more about the High Resolution Melting range or any of our other products, feel free to reach out to our team today.

What Our Customers Say

First time, all the time!

The product itself worked amazingly for PCR and more than pays for itself – enabling first time results and therefore cutting reaction costs by using less master mix. After-sales care absolutely second to none. We are now looking to completely move over to the Microzone range.

~ Melissa R.