MegaMix Gold Mastermix

Description

MegaMix Gold – Hot Start PCR Mastermix

The first few cycles of PCR are always the most important to successful amplification. We ensure that our mastermixes are never short of enough kick to start the first few cycles and powerfully continue your amplification. MegaMix Gold uses a superior, sensitive, hot start DNA polymerase which becomes active upon heating at 95C. This provides a highly specific and sensitive amplification without background or primer dimer formation. Our 2x mix contains 400 µM dNTP and 6 mM MgCl₂ in Microzone’s proprietary PCR enhancing buffer.

Using Microzone’s own proprietary engineered PCR buffers, our mastermixes provide powerful, broad template amplification, giving the user confidence. PCR products generated are A-tailed and suitable for ligating into TA cloning vectors.

MegaMix – by Microzone

Microzone’s MegaMix range of PCR mastermixes covers a broad range of a scientist’s requirements. We offer a MegaMix for most occasions including:

• Routine End Point PCR
• Hot Start End Point PCR
• qPCR
• RT-qPCR
• High Fidelity (HiFi) PCR
• Inhibitor Resistant PCR
• High Resolution Melting
• RT-PCR

Associated products include – Taq’s with buffer; dNTP mixes; Molecular Grade Water…….