Crude PCR

Crude PCR enables the amplification of DNA directly from unprocessed or minimally processed samples, providing a cost-effective and time-saving alternative to traditional workflows. This method minimizes sample loss and is particularly beneficial for high-throughput applications such as genotyping, transgene detection, knockout analysis, and PCR-based clinical and forensic testing.

One of the main challenges with crude PCR is the presence of inhibitors that can affect reaction sensitivity, yield, and specificity. Microzone addresses these challenges with a range of direct-to-PCR lysis buffers and inhibitor-resistant mastermixes, including MegaMix Diamond and MegaMix Ruby, which are designed to ensure reliable and efficient amplification even from complex samples.

These solutions are compatible with diverse sample types, including buccal swabs, hair follicles, mouse tails, blood, bacteria, larvae, and fungi. By integrating efficient DNA release and direct-to-PCR amplification workflows, Microzone provides a seamless approach to crude sample preparation and DNA analysis, delivering robust and accurate results with minimal handling.