PCR Product Clean-Up

PCR product clean-up is a critical step in molecular biology workflows, as it ensures the removal of residual primers, nucleotides, enzymes, and other reaction components that could interfere with downstream applications like sequencing, cloning, and quantitative PCR. Without proper clean-up, contaminants can inhibit reactions, lead to low-quality results, or cause failed experiments. Clean-up ensures that the DNA is purified, ready for accurate and reliable analysis in sensitive applications.

Microzone offers three effective solutions for PCR product purification: microCLEAN and MAGneat PCR Beads, along with microCLEAN XL for larger PCR amplicons.

microCLEAN & microCLEAN XL

microCLEAN is suitable for amplicons up to 1 kb and is a fast, efficient clean-up solution that uses proprietary buffer technology to bind DNA directly to the tube wall, eliminating the need for spin columns or magnetic racks. This process takes less than 20 minutes, simplifying purification by combining the binding and washing steps into a single procedure. It offers high yields and purity, making it ideal for a variety of downstream applications such as sequencing, cloning, and restriction digestion. microCLEAN is cost-effective and environmentally friendly, as it reduces plastic waste and eliminates the need for solvents.

For PCR amplicons larger than 1 kb, microCLEAN XL provides the same efficient clean-up process but is specially designed to handle larger DNA fragments. This solution ensures effective purification of even larger amplicons while maintaining high yield and purity, making it a versatile, time-saving option for labs working with longer PCR products.

MAGneat PCR Beads

MAGneat PCR Beads use magnetic bead-based purification, providing high-yield and high-purity clean-up. This method is well-suited for high-throughput or sensitive applications such as next-generation sequencing (NGS). The magnetic beads capture DNA, efficiently removing contaminants and ensuring the highest DNA quality for downstream applications. The ratio of beads to sample can be manipulated allowing the different size amplicons to selectively purified. Additionally, MAGneat PCR Beads can be automated, making it ideal for large-scale processing.

Choosing the Right Solution

For routine clean-up of moderate sample volumes, microCLEAN is the best option, offering simplicity, cost-effectiveness, and high recovery. When working with larger PCR amplicons, microCLEAN XL is specially optimized for those needs while maintaining ease of use. For high-throughput applications or when maximum DNA purity is critical, MAGneat PCR Beads is the optimal choice.

microCLEAN, microCLEAN XL, and MAGneat PCR Beads all provide reliable and efficient clean-up solutions, ensuring high-quality DNA ready for any downstream PCR-based applications.