Sample Preparation for Sanger Sequencing

Sanger sequencing remains a cornerstone of DNA analysis, requiring high-quality templates for accurate and reliable results. It is a method of determining the precise order of nucleotides in a DNA molecule using chain-terminating dideoxynucleotides during DNA synthesis.

Efficient sample preparation is critical to remove impurities that may interfere with sequencing reactions and to ensure robust, high-resolution data. Microzone’s Free From range offers streamlined solutions for preparing samples, from DNA extraction to amplification and purification, simplifying workflows while delivering reliable outcomes.

Streamlined Workflow with the Free From Range

Microzone’s Free From range provides a complete solution for preparing samples for Sanger sequencing:

• microLYSIS Plus offers a rapid, thermal lysis-based method to extract DNA directly from crude samples such as tissues, blood, or bacteria in as little as 15 minutes. This eliminates labour-intensive purification steps while maintaining DNA integrity.
• MegaMix Crystal Q5 High Fidelity ensures robust and high-quality amplification of DNA from crude lysates. This advanced mastermix tolerates inhibitors and delivers precise, high-fidelity PCR products suitable for Sanger sequencing.
• microCLEAN provides a quick and efficient clean-up solution for PCR amplicons. This single-tube method simplifies the removal of contaminants such as primers, dNTPs, and enzymes, delivering high-yield DNA ideal for downstream sequencing applications.

Simplified and Efficient Sample Preparation

The combination of microLYSIS Plus, MegaMix Crystal Q5 High Fidelity, and microCLEAN streamlines the preparation of sequencing-ready samples. These products eliminate the need for complex, time-consuming extraction and purification protocols, enabling rapid processing of even challenging samples.

Key Benefits

• Time-Saving: Rapid extraction, amplification, and purification processes significantly reduce preparation time.
• High-Quality Results: Ensures DNA templates and PCR products are sequencing-ready, even from crude or complex samples.
• Cost-Effective: Minimizes the need for expensive purification kits and complex workflows, offering a streamlined and affordable solution.

Microzone’s Free From range delivers an efficient and reliable approach to sample preparation for Sanger sequencing, supporting researchers in achieving accurate results with ease.