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2MME-RTU
MegaMix Emerald Dye RT-qPCR Kit
£101.00 – £825.00
Description
MegaMix Emerald Dye RT-qPCR Kit
Containing all the components needed to perform RT-qPCR swiftly and reliably.
MegaMix Emerald uses Microzone’s third generation, double stranded DNA intercalating fluorescent dye, microGREEN. MegaMix Emerald is the perfect choice for qPCR, Melt Curve Analysis and High Resolution Melting (HRM). The Hot Start Taq polymerase is chemically inactivated until heating to 95°C, providing excellent sensitivity and specificity; eliminating the formation of non-specific amplification and primer-dimers. The presence of UNG and dUTP’s eliminates carryover contamination, the thermolabile UNG is active at room temperature before being completely and irreversibly inactivated when heated to 95°C, this means your PCR product is suitable for downstream processing.
MegaMix – by Microzone
Microzone’s MegaMix range of PCR mastermixes covers a broad range of a scientist’s requirements. We offer a MegaMix for most occasions including:
- Routine End Point PCR
- Hot Start End Point PCR
- qPCR
- RT-qPCR
- High Fidelity (HiFi) PCR
- Inhibitor Resistant PCR
- High Resolution Melting
- RT-PCR
Associated products include – Taq’s with buffer; dNTP mixes; Molecular Grade Water…….
Additional information
| Quantity | 100 Reactions, 200 Reactions, 1000 Reactions |
|---|---|
| ROX | No-ROX, Lo-ROX, Hi-ROX |
MegaMix Emerald Dye RT-qPCR Kit
Containing all the components needed to perform RT-qPCR swiftly and reliably.
MegaMix Emerald uses Microzone's third generation, double stranded DNA intercalating fluorescent dye, microGREEN. MegaMix Emerald is the perfect choice for qPCR, Melt Curve Analysis and High Resolution Melting (HRM). The Hot Start Taq polymerase is chemically inactivated until heating to 95°C, providing excellent sensitivity and specificity; eliminating the formation of non-specific amplification and primer-dimers. The presence of UNG and dUTP’s eliminates carryover contamination, the thermolabile UNG is active at room temperature before being completely and irreversibly inactivated when heated to 95°C, this means your PCR product is suitable for downstream processing.
MegaMix - by Microzone
Microzone's MegaMix range of PCR mastermixes covers a broad range of a scientist's requirements. We offer a MegaMix for most occasions including:
- Routine End Point PCR
- Hot Start End Point PCR
- qPCR
- RT-qPCR
- High Fidelity (HiFi) PCR
- Inhibitor Resistant PCR
- High Resolution Melting
- RT-PCR
Associated products include - Taq's with buffer; dNTP mixes; Molecular Grade Water.......
- Incorporates Microzone’s proprietary third generation, none-inhibitory intercalating dye, microGREEN, for increased fluorescence and unmatched sensitivity.
- Confidence inspiring, incorporation of thermolabile UNG and dUTP’s prevent carryover contamination and allow for use in downstream applications.
- Compatible with any qPCR instrument with a FAM/SYBR channel.
- One-Step format slashes time and effort required to get reliable results.
Applications include:
- Quantification of any RNA template (mRNA, total RNA, viral RNA), low copy number samples.
- Gene expression analysis.
- Pathogen detection.
- RT-qPCR assays using fluorescence of intercalating dye.
- High resolution melting.
The kit consists of
- 2X MegaMix Emerald Mastermix, containingHot Start Taq DNA polymerase, Microzone’s intercalating dye microGREEN, dUTP and UNG in enhancing buffer.
- 20X RTase/RI enzyme mix, optimised for amplifying low copy RNA targets.
- Just Water, our molecular biology grade water.
Available in 200 & 1000 reactions.
- Incorporates Microzone’s proprietary third generation, none-inhibitory intercalating dye, microGREEN, for increased fluorescence and unmatched sensitivity.
- Confidence inspiring, incorporation of thermolabile UNG and dUTP’s prevent carryover contamination and allow for use in downstream applications.
- Compatible with any qPCR instrument with a FAM/SYBR channel.
- One-Step format slashes time and effort required to get reliable results.
Applications include:
- Quantification of any RNA template (mRNA, total RNA, viral RNA), low copy number samples.
- Gene expression analysis.
- Pathogen detection.
- RT-qPCR assays using fluorescence of intercalating dye.
- High resolution melting.
The kit consists of
- 2X MegaMix Emerald Mastermix, containingHot Start Taq DNA polymerase, Microzone’s intercalating dye microGREEN, dUTP and UNG in enhancing buffer.
- 20X RTase/RI enzyme mix, optimised for amplifying low copy RNA targets.
- Just Water, our molecular biology grade water.
Available in 200 & 1000 reactions.
Fast Delivery
Order before 12:00 GMT, dispatched today.
Dedicated Support
Our team is always on hand to provide assistance.
Satisfaction Guarantee
Join over 2000 satisfied Microzone customers.
David, UK
Custom Orders Made Easy
At Microzone, we recognise the unique demands of every laboratory. Whether you're ramping up experiments or seeking specialised formulations, our dedicated team is on standby. Contact us today and benefit from personalised attention that ensures your order is precisely tailored.
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