Your basket is currently empty!
PK-1
Proteinase K
£43.00
Description
Microzone’s Proteinase K, a highly active serine protease derived from Tritirachium album, is designed to degrade proteins efficiently across a broad range of conditions. With a concentration of 20 mg/mL, this solution is ready to use and requires no reconstitution, offering convenience and flexibility for a variety of protocols. Known for its stability in high temperatures, varying pH levels, and denaturing conditions, Proteinase K is ideal for applications including nucleic acid extraction, DNase/RNase inactivation, and in situ hybridization.
Additional information
| Quantity | 1mL |
|---|
Microzone’s Proteinase K, a highly active serine protease derived from Tritirachium album, is designed to degrade proteins efficiently across a broad range of conditions. With a concentration of 20 mg/mL, this solution is ready to use and requires no reconstitution, offering convenience and flexibility for a variety of protocols. Known for its stability in high temperatures, varying pH levels, and denaturing conditions, Proteinase K is ideal for applications including nucleic acid extraction, DNase/RNase inactivation, and in situ hybridization.
- High Activity: Effective protein degradation for a wide range of applications.
- Broad Specificity: Cleaves peptide bonds near aliphatic and aromatic amino acids.
- Stability: Maintains activity over a broad pH range (4.0–12.5) and temperatures (up to 65°C).
- Ready-to-Use: Supplied in a 20 mg/mL solution for ease of application.
- Nucleic Acid Extraction (DNA/RNA Isolation)
- Enzyme Inactivation (DNase/RNase)
- In Situ Hybridization (Enhances probe accessibility by digesting protein barriers)
- Concentration: 20 mg/mL serine protease solution.
- Optimal Conditions: pH 8.0; 50-60°C for increased activity.
- Activators: Requires 1–5 mM Ca²⁺ for enhanced stability and activity.
- Inhibitors: Inhibited by DIFP or PMSF at 5 mM concentration.
- Storage: Store as recommended to preserve stability and limit light exposure.
- High Activity: Effective protein degradation for a wide range of applications.
- Broad Specificity: Cleaves peptide bonds near aliphatic and aromatic amino acids.
- Stability: Maintains activity over a broad pH range (4.0–12.5) and temperatures (up to 65°C).
- Ready-to-Use: Supplied in a 20 mg/mL solution for ease of application.
- Nucleic Acid Extraction (DNA/RNA Isolation)
- Enzyme Inactivation (DNase/RNase)
- In Situ Hybridization (Enhances probe accessibility by digesting protein barriers)
- Concentration: 20 mg/mL serine protease solution.
- Optimal Conditions: pH 8.0; 50-60°C for increased activity.
- Activators: Requires 1–5 mM Ca²⁺ for enhanced stability and activity.
- Inhibitors: Inhibited by DIFP or PMSF at 5 mM concentration.
- Storage: Store as recommended to preserve stability and limit light exposure.
Fast Delivery
Order before 12:00 GMT, dispatched today.
Dedicated Support
Our team is always on hand to provide assistance.
Satisfaction Guarantee
Join over 2000 satisfied Microzone customers.
David, UK
Custom Orders Made Easy
At Microzone, we recognise the unique demands of every laboratory. Whether you're ramping up experiments or seeking specialised formulations, our dedicated team is on standby. Contact us today and benefit from personalised attention that ensures your order is precisely tailored.
Contact Sales TeamRelated products
-
MAGneat Magnetic Separation Racks
2MSR £125.00 – £145.00A range of separation racks designed with Neodymium magnets.
-
Just Water – PCR Grade Water
5JWA £34.50PCR grade water for all you requirements.
-
dNTP Mix
DNTP £102.00 – £219.50High-purity dNTP mix for efficient DNA synthesis in PCR and other molecular biology applications.
