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MegaMix Platinum - Probe based qPCR Mastermix
Containing all the components needed to perform multiplex qPCR swiftly and reliably. The 2X mix contains chemically modified Hot Start Taq DNA polymerase, dUTP and UNG in Microzone’s proprietary enhancing buffer optimised for amplifying low copy DNA targets.
The presence of thermolabile UNG and dUTP eliminates carryover contamination at room temperature. Unlike other UNG enzymes it is totally and irreversibly inactivated during thermocycling allowing post PCR analysis.
This mix has been optimized for multiplex assays utilising dual labelled hydrolysis probes such as TaqMan and Scorpion®.
MegaMix Platinum is compatible with both standard and fast cycling conditions.
An excellent mix for anyone looking to create probe based qPCR based kits.
MegaMix - by Microzone
Microzone's MegaMix range of PCR mastermixes covers a broad range of a scientist's requirements. We offer a MegaMix for most occasions including:
- Routine End Point PCR
- Hot Start End Point PCR
- qPCR
- RT-qPCR
- High Fidelity (HiFi) PCR
- Inhibitor Resistant PCR
- High Resolution Melting
- RT-PCR
Associated products include - Taq's with buffer; dNTP mixes; Molecular Grade Water.......
- Robust linear amplification of multiple DNA targets for probe based detection.
- Confidence inspiring, incorporation of thermolabile UNG and dUTP’s prevent
carryover contamination and allow for use in downstream applications.
- Designed for use with probe based assays using TaqMan, Scorpions, Molecular Beacons etc.
- Detection and quantification of gDNA, cDNA, Viral DNA etc.
- Excellent for multiplex assays.
- Robust linear amplification of multiple DNA targets for probe based detection.
- Confidence inspiring, incorporation of thermolabile UNG and dUTP’s prevent
carryover contamination and allow for use in downstream applications.
- Designed for use with probe based assays using TaqMan, Scorpions, Molecular Beacons etc.
- Detection and quantification of gDNA, cDNA, Viral DNA etc.
- Excellent for multiplex assays.
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David, UK
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