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2MMP
MegaMix Platinum qPCR Mastermix (Probe Based)
£101.00 – £450.00
Description
MegaMix Platinum – Probe based qPCR Mastermix
Containing all the components needed to perform multiplex qPCR swiftly and reliably. The 2X mix contains chemically modified Hot Start Taq DNA polymerase, dUTP and UNG in Microzone’s proprietary enhancing buffer optimised for amplifying low copy DNA targets.
The presence of thermolabile UNG and dUTP eliminates carryover contamination at room temperature. Unlike other UNG enzymes it is totally and irreversibly inactivated during thermocycling allowing post PCR analysis.
This mix has been optimized for multiplex assays utilising dual labelled hydrolysis probes such as TaqMan and Scorpion®.
MegaMix Platinum is compatible with both standard and fast cycling conditions.
An excellent mix for anyone looking to create probe based qPCR based kits.
High Temperature Stability – MegaMix Platinum was stored at 40ËšC over a period of 34 days. The product demonstrated the same activity using a triplex assay using TaqMan probes on day 34 as it did on day 1. Data shown in carousel. This supports the use of MegaMix Platinum in hot environments where cold storage may be difficult to attain.
MegaMix – by Microzone
Microzone’s MegaMix range of PCR mastermixes covers a broad range of a scientist’s requirements. We offer a MegaMix for most occasions including:
- Routine End Point PCR
- Hot Start End Point PCR
- qPCR
- RT-qPCR
- High Fidelity (HiFi) PCR
- Inhibitor Resistant PCR
- High Resolution Melting
- RT-PCR
Associated products include – Taq’s with buffer; dNTP mixes; Molecular Grade Water…….
Additional information
| Quantity | 200 Reactions, 1000 Reactions |
|---|---|
| ROX | No-ROX, Lo-ROX, Hi-ROX |
MegaMix Platinum - Probe based qPCR Mastermix
Containing all the components needed to perform multiplex qPCR swiftly and reliably. The 2X mix contains chemically modified Hot Start Taq DNA polymerase, dUTP and UNG in Microzone’s proprietary enhancing buffer optimised for amplifying low copy DNA targets.
The presence of thermolabile UNG and dUTP eliminates carryover contamination at room temperature. Unlike other UNG enzymes it is totally and irreversibly inactivated during thermocycling allowing post PCR analysis.
This mix has been optimized for multiplex assays utilising dual labelled hydrolysis probes such as TaqMan and Scorpion®.
MegaMix Platinum is compatible with both standard and fast cycling conditions.
An excellent mix for anyone looking to create probe based qPCR based kits.
High Temperature Stability - MegaMix Platinum was stored at 40˚C over a period of 34 days. The product demonstrated the same activity using a triplex assay using TaqMan probes on day 34 as it did on day 1. Data shown in carousel. This supports the use of MegaMix Platinum in hot environments where cold storage may be difficult to attain.
MegaMix - by Microzone
Microzone's MegaMix range of PCR mastermixes covers a broad range of a scientist's requirements. We offer a MegaMix for most occasions including:
- Routine End Point PCR
- Hot Start End Point PCR
- qPCR
- RT-qPCR
- High Fidelity (HiFi) PCR
- Inhibitor Resistant PCR
- High Resolution Melting
- RT-PCR
Associated products include - Taq's with buffer; dNTP mixes; Molecular Grade Water.......
Standard Curve of MegaMix Platinum
qPCR_MMP_MRSA_MecC_Amplification Curve_1x10'11
MegaMix Platinum stored at 40ËšC in a controlled manner. Test were performed using a triplex assay over a period of 34 days. As can be seen at day 34 the results we comparable to day 1. Performance of MegaMix Platinum remained solid throughout the period tested. Testing was stopped at day 34.
- Robust linear amplification of multiple DNA targets for probe based detection.
- Confidence inspiring, incorporation of thermolabile UNG and dUTP’s prevent
carryover contamination and allow for use in downstream applications.
- Designed for use with probe based assays using TaqMan, Scorpions, Molecular Beacons etc.
- Detection and quantification of gDNA, cDNA, Viral DNA etc.
- Excellent for multiplex assays.
- Robust linear amplification of multiple DNA targets for probe based detection.
- Confidence inspiring, incorporation of thermolabile UNG and dUTP’s prevent
carryover contamination and allow for use in downstream applications.
- Designed for use with probe based assays using TaqMan, Scorpions, Molecular Beacons etc.
- Detection and quantification of gDNA, cDNA, Viral DNA etc.
- Excellent for multiplex assays.
Fast Delivery
Order before 12:00 GMT, dispatched today.
Dedicated Support
Our team is always on hand to provide assistance.
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Join over 2000 satisfied Microzone customers.
David, UK
Custom Orders Made Easy
At Microzone, we recognise the unique demands of every laboratory. Whether you're ramping up experiments or seeking specialised formulations, our dedicated team is on standby. Contact us today and benefit from personalised attention that ensures your order is precisely tailored.
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