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The SwiftScript cDNA Synthesis Kit enables efficient first-strand cDNA synthesis from diverse RNA templates, including complex secondary structures. With a thermostable reverse transcriptase and RNase inhibitors, SwiftScript is designed for rapid, high-yield cDNA production without the need for extensive optimization. Its reaction buffer is optimized with oligo(dT) and random primers, ensuring unbiased, consistent synthesis suitable for gene expression analysis, labeled-cDNA probe generation, and cDNA library construction.
- Unbiased Synthesis: Compatible with complex RNA templates, including GC-rich regions.
- Rapid Process: Completes synthesis in 25 minutes without RNA denaturation.
- Enhanced Priming: Anchored oligo(dT) and random hexamers for robust priming.
- Optimized Buffer: High specificity and yields for downstream applications.
- cDNA synthesis from mRNA, total RNA, and viral RNA
- Gene expression analysis
- RT-PCR for labeled-cDNA probes
- cDNA library construction
- Reaction Buffer: 5X concentration with dNTPs, primers, and enhancers.
- Enzyme Mix: 20X SwiftScript Enzyme Mix with reverse transcriptase and RNase inhibitors.
- Formats: Available for 50 reactions.
- Storage: Store as recommended to maintain stability and enzyme activity.
- Unbiased Synthesis: Compatible with complex RNA templates, including GC-rich regions.
- Rapid Process: Completes synthesis in 25 minutes without RNA denaturation.
- Enhanced Priming: Anchored oligo(dT) and random hexamers for robust priming.
- Optimized Buffer: High specificity and yields for downstream applications.
- cDNA synthesis from mRNA, total RNA, and viral RNA
- Gene expression analysis
- RT-PCR for labeled-cDNA probes
- cDNA library construction
- Reaction Buffer: 5X concentration with dNTPs, primers, and enhancers.
- Enzyme Mix: 20X SwiftScript Enzyme Mix with reverse transcriptase and RNase inhibitors.
- Formats: Available for 50 reactions.
- Storage: Store as recommended to maintain stability and enzyme activity.
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