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2MMB
MegaMix Blue PCR Mastermix
£17.00 – £1,210.00
Description
MegaMix Blue End Point PCR Mastermix:
One of Microzone’s first MegaMixes. Over the years this MegaMix has stood tall. It’s still a firm favourite with many customers saying it’s their go to end point PCR mastermix.
From the start we knew that the first few cycles of PCR are always the most important to successful amplification. Therefore, our mastermixes are never short of enough polymerase to kick start and then powerfully continue your amplification. MegaMix Blue (and MegaMix) are designed to cope with whatever is thrown at them. We incorporate two different Taq polymerases. The two Taqs complement each other and offer customers the capability to cover a large range of templates with just one mastermix. The addition of Microzone’s own proprietary engineered PCR buffer ensures that our mastermixes provide powerful, broad template, amplification.
Looking for a non-hot start PCR mastermix? You can’t go far wrong with our tried and fully tested over many years MegaMix Blue or MegaMix PCR mastermixes.
MegaMix – by Microzone
Microzone’s MegaMix range of PCR mastermixes covers a broad range of a scientist’s requirements. We offer a MegaMix for most occasions including:
- Routine End Point PCR
- Hot Start End Point PCR
- qPCR
- RT-qPCR
- High Fidelity (HiFi) PCR
- Inhibitor Resistant PCR
- High Resolution Melting
- RT-PCR
Associated products include – Taq’s with buffer; dNTP mixes; Molecular Grade Water…….
Additional information
| Concentration | Standard, Double |
|---|---|
| Quantity | 1mL, 5 X 1 mL, 25 X 1 mL, 100 X 1 mL, 1mL, 5 X 0.5 mL, 25 X 0.5 mL, 100 X 0.5 mL |
MegaMix Blue End Point PCR Mastermix:
One of Microzone's first MegaMixes. Over the years this MegaMix has stood tall. It's still a firm favourite with many customers saying it's their go to end point PCR mastermix.
From the start we knew that the first few cycles of PCR are always the most important to successful amplification. Therefore, our mastermixes are never short of enough polymerase to kick start and then powerfully continue your amplification. MegaMix Blue (and MegaMix) are designed to cope with whatever is thrown at them. We incorporate two different Taq polymerases. The two Taqs complement each other and offer customers the capability to cover a large range of templates with just one mastermix. The addition of Microzone’s own proprietary engineered PCR buffer ensures that our mastermixes provide powerful, broad template, amplification.
Looking for a non-hot start PCR mastermix? You can't go far wrong with our tried and fully tested over many years MegaMix Blue or MegaMix PCR mastermixes.
MegaMix - by Microzone
Microzone's MegaMix range of PCR mastermixes covers a broad range of a scientist's requirements. We offer a MegaMix for most occasions including:
- Routine End Point PCR
- Hot Start End Point PCR
- qPCR
- RT-qPCR
- High Fidelity (HiFi) PCR
- Inhibitor Resistant PCR
- High Resolution Melting
- RT-PCR
Associated products include - Taq's with buffer; dNTP mixes; Molecular Grade Water.......
MegaMix Blue MasterMix exhibits excellent product yields and sensitivity. 33 ng of human genomic DNA was serially diluted 3-fold eight times and amplified with MegaMix Blue Mastermix using HFE1 primer set (390 bp product). From left to right: PCR product generated from 33 ng to 0.6 pg of hgDNA per reaction. M = 100 bp DNA ladder.
- Specially formulated Taq polymerase blend in optimised buffer for efficient PCR
amplification. - Inert blue loading dye for direct gel loading.
- Available as MegaMix Blue (1x Conc) and MegaMix Blue Double (2x Conc)
- Routine end point PCR.
- Fast PCR.
- Genotyping.
- Amplification of GC and AT rich templates.
- Up to 6kb.
- Methylated DNA.
- TA Cloning
Cycling Profile (guide only)
- Initial denaturation step: 95⁰C for 3 mins
- Then Cycle 25-30 times:
- Step 1: 95⁰C for 30 secs
- Step 2: Optimal annealing temp. of primers for 30 to 60 secs
- Step 3: 72⁰C for 45 to 60 secs
- Cool to room temperature
- Thaw reagents prior to use. Mix well prior to use.
- GC and complex templates may require higher denaturation temperatures
- Two step cycling protocols can increase speed. Following annealing just ramp the temperature back to denaturation.
- If using new primers or changing your master mix it will be necessary to perform a new annealing temperature gradient. We suggest 1⁰C intervals and to start at 5⁰C below the calculated Tm of the primers and go to 5⁰C above the primer Tm.
- Primers design and quality have a large impact on the reaction. Taking time to design your primers using good techniques or software will benefit down the line. We do offer a technical note on primer design. Contact us to receive a copy.
- Specially formulated Taq polymerase blend in optimised buffer for efficient PCR
amplification. - Inert blue loading dye for direct gel loading.
- Available as MegaMix Blue (1x Conc) and MegaMix Blue Double (2x Conc)
- Routine end point PCR.
- Fast PCR.
- Genotyping.
- Amplification of GC and AT rich templates.
- Up to 6kb.
- Methylated DNA.
- TA Cloning
Cycling Profile (guide only)
- Initial denaturation step: 95⁰C for 3 mins
- Then Cycle 25-30 times:
- Step 1: 95⁰C for 30 secs
- Step 2: Optimal annealing temp. of primers for 30 to 60 secs
- Step 3: 72⁰C for 45 to 60 secs
- Cool to room temperature
- Thaw reagents prior to use. Mix well prior to use.
- GC and complex templates may require higher denaturation temperatures
- Two step cycling protocols can increase speed. Following annealing just ramp the temperature back to denaturation.
- If using new primers or changing your master mix it will be necessary to perform a new annealing temperature gradient. We suggest 1⁰C intervals and to start at 5⁰C below the calculated Tm of the primers and go to 5⁰C above the primer Tm.
- Primers design and quality have a large impact on the reaction. Taking time to design your primers using good techniques or software will benefit down the line. We do offer a technical note on primer design. Contact us to receive a copy.
Fast Delivery
Order before 12:00 GMT, dispatched today.
Dedicated Support
Our team is always on hand to provide assistance.
Satisfaction Guarantee
Join over 2000 satisfied Microzone customers.
David, UK
Custom Orders Made Easy
At Microzone, we recognise the unique demands of every laboratory. Whether you're ramping up experiments or seeking specialised formulations, our dedicated team is on standby. Contact us today and benefit from personalised attention that ensures your order is precisely tailored.
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