2MMG

MegaMix Gold Mastermix

£54.00£4,499.00

Description

MegaMix Gold – Hot Start PCR Mastermix

The first few cycles of PCR are always the most important to successful amplification. We ensure that our mastermixes are never short of enough kick to start the first few cycles and powerfully continue your amplification. MegaMix Gold uses a superior, sensitive, hot start DNA polymerase which becomes active upon heating at 95C. This provides a highly specific and sensitive amplification without background or primer dimer formation. Our 2x mix contains 400 µM dNTP and 6 mM MgCl2 in Microzone’s proprietary PCR enhancing buffer.

Using Microzone’s own proprietary engineered PCR buffers, our mastermixes provide powerful, broad template amplification, giving the user confidence. PCR products generated are A-tailed and suitable for ligating into TA cloning vectors.

MegaMix – by Microzone

Microzone’s MegaMix range of PCR mastermixes covers a broad range of a scientist’s requirements. We offer a MegaMix for most occasions including:

  • Routine End Point PCR
  • Hot Start End Point PCR
  • qPCR
  • RT-qPCR
  • High Fidelity (HiFi) PCR
  • Inhibitor Resistant PCR
  • High Resolution Melting
  • RT-PCR

Associated products include – Taq’s with buffer; dNTP mixes; Molecular Grade Water…….

 

Additional information

Quantity

1 mL, 5 X 1 mL, 10 X 1 mL, 50 X 1 mL, 100 X 1 mL


MegaMix Gold - Hot Start PCR Mastermix

The first few cycles of PCR are always the most important to successful amplification. We ensure that our mastermixes are never short of enough kick to start the first few cycles and powerfully continue your amplification. MegaMix Gold uses a superior, sensitive, hot start DNA polymerase which becomes active upon heating at 95C. This provides a highly specific and sensitive amplification without background or primer dimer formation. Our 2x mix contains 400 µM dNTP and 6 mM MgCl2 in Microzone’s proprietary PCR enhancing buffer.

Using Microzone’s own proprietary engineered PCR buffers, our mastermixes provide powerful, broad template amplification, giving the user confidence. PCR products generated are A-tailed and suitable for ligating into TA cloning vectors.

MegaMix - by Microzone

Microzone's MegaMix range of PCR mastermixes covers a broad range of a scientist's requirements. We offer a MegaMix for most occasions including:

  • Routine End Point PCR
  • Hot Start End Point PCR
  • qPCR
  • RT-qPCR
  • High Fidelity (HiFi) PCR
  • Inhibitor Resistant PCR
  • High Resolution Melting
  • RT-PCR

Associated products include - Taq's with buffer; dNTP mixes; Molecular Grade Water.......

 

  • Small molecule mediated Hot-Start prevents non-specific amplification and primer
    dimer formation.
  • Amplify products up to 6kb.
  • High yields under standard conditions.
  • Provides effective and efficient amplification for GC and AT rich templates.
  • Strong MgCl2 to support strong amplification.
  • Excellent mix for single plex qPCR for both dye and probe based methods
  • Exceptionally stable and can be freeze thawed on multiple occasions.
  • Microzone PCR Buffer – provides increased stability, performance and ease of use.
  • Tailored for those intricate PCR systems that demand nothing but the best
  • Ideal for professionals seeking consistent, top-tier outcomes in their PCR workflows

Contents: Elite Hot Start PCR Master Mix
Concentration: Double concentrated for peak performance
Packaging: Multiple acquisition options, with bulk purchases available upon inquiry

Megamix Gold Protocol

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Join over 2000 satisfied Microzone customers.

David, UK

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