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MegaMix Gold Mastermix
£54.00 – £4,499.00
Description
MegaMix Gold – Hot Start PCR Mastermix
The first few cycles of PCR are always the most important to successful amplification. We ensure that our mastermixes are never short of enough kick to start the first few cycles and powerfully continue your amplification. MegaMix Gold uses a superior, sensitive, hot start DNA polymerase which becomes active upon heating at 95C. This provides a highly specific and sensitive amplification without background or primer dimer formation. Our 2x mix contains 400 µM dNTP and 6 mM MgCl2 in Microzone’s proprietary PCR enhancing buffer.
Using Microzone’s own proprietary engineered PCR buffers, our mastermixes provide powerful, broad template amplification, giving the user confidence. PCR products generated are A-tailed and suitable for ligating into TA cloning vectors.
MegaMix – by Microzone
Microzone’s MegaMix range of PCR mastermixes covers a broad range of a scientist’s requirements. We offer a MegaMix for most occasions including:
- Routine End Point PCR
- Hot Start End Point PCR
- qPCR
- RT-qPCR
- High Fidelity (HiFi) PCR
- Inhibitor Resistant PCR
- High Resolution Melting
- RT-PCR
Associated products include – Taq’s with buffer; dNTP mixes; Molecular Grade Water…….
Additional information
| Quantity | 1 mL, 5 X 1 mL, 10 X 1 mL, 50 X 1 mL, 100 X 1 mL |
|---|
MegaMix Gold - Hot Start PCR Mastermix
The first few cycles of PCR are always the most important to successful amplification. We ensure that our mastermixes are never short of enough kick to start the first few cycles and powerfully continue your amplification. MegaMix Gold uses a superior, sensitive, hot start DNA polymerase which becomes active upon heating at 95C. This provides a highly specific and sensitive amplification without background or primer dimer formation. Our 2x mix contains 400 µM dNTP and 6 mM MgCl2 in Microzone’s proprietary PCR enhancing buffer.
Using Microzone’s own proprietary engineered PCR buffers, our mastermixes provide powerful, broad template amplification, giving the user confidence. PCR products generated are A-tailed and suitable for ligating into TA cloning vectors.
MegaMix - by Microzone
Microzone's MegaMix range of PCR mastermixes covers a broad range of a scientist's requirements. We offer a MegaMix for most occasions including:
- Routine End Point PCR
- Hot Start End Point PCR
- qPCR
- RT-qPCR
- High Fidelity (HiFi) PCR
- Inhibitor Resistant PCR
- High Resolution Melting
- RT-PCR
Associated products include - Taq's with buffer; dNTP mixes; Molecular Grade Water.......
MegaMix Gold MasterMix exhibits excellent product yields and sensitivity. 33 ng of human genomic DNA was serially diluted 3-fold nine times and amplified with MegaMix Gold Mastermix using HFE1 primer set (390 bp product). From left to right: PCR product generated from 33 ng to 0.2 pg of hgDNA per reaction. M = 100 bp DNA ladder.
MegaMix Gold MasterMix exhibits great performance and reproducibility in real-time PCR. 70 ng of human genomic DNA was serially diluted 10-fold four times and amplified with MegaMix Gold Mastermix and microGREEN dye using RNase P primer set (65 bp product). The melting curves for each amplification are shown in the inset. Graph was plotted and analysed with micPCR software (Bio Molecular Systems, Upper Coomera – Australia).
- Small molecule mediated Hot-Start prevents non-specific amplification and primer
dimer formation. - Amplify products up to 6kb.
- High yields under standard conditions.
- Provides effective and efficient amplification for GC and AT rich templates.
- Strong MgCl2 to support strong amplification.
- Tailored for those intricate PCR systems that demand nothing but the best
- Ideal for professionals seeking consistent, top-tier outcomes in their PCR workflows
Contents: Elite Hot Start PCR Master Mix
Concentration: Double concentrated for peak performance
Packaging: Multiple acquisition options, with bulk purchases available upon inquiry
- Small molecule mediated Hot-Start prevents non-specific amplification and primer
dimer formation. - Amplify products up to 6kb.
- High yields under standard conditions.
- Provides effective and efficient amplification for GC and AT rich templates.
- Strong MgCl2 to support strong amplification.
- Tailored for those intricate PCR systems that demand nothing but the best
- Ideal for professionals seeking consistent, top-tier outcomes in their PCR workflows
Contents: Elite Hot Start PCR Master Mix
Concentration: Double concentrated for peak performance
Packaging: Multiple acquisition options, with bulk purchases available upon inquiry
Fast Delivery
Order before 12:00 GMT, dispatched today.
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At Microzone, we recognise the unique demands of every laboratory. Whether you're ramping up experiments or seeking specialised formulations, our dedicated team is on standby. Contact us today and benefit from personalised attention that ensures your order is precisely tailored.
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