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2MMEU
MegaMix Emerald qPCR Mastermix (Dye Based)
£56.00 – £450.00
Description
MegaMix Emerald dye based qPCR Mastermix
Containing all the components needed to perform dye based qPCR swiftly and reliably. The 2X mix contains chemically modified Hot Start Taq DNA polymerase, intercalating dye, dUTP and UNG in enhancing Microzone’s proprietary PCR buffer optimised for amplifying low copy DNA targets.
We have incorporated MicroGREEN Dye ( a third generation intercalating highly fluorescent dye that binds to double stranded DNA) in to MegaMix Emerald. This dye mix combinations makes MegaMix Emerald the perfect choice for qPCR, Melt Curve Analysis and High Resolution Melting (HRM).
The Hot Start Taq polymerase is chemically inactivated until heating to 95°C, providing excellent sensitivity and specificity; eliminating the formation of non-specific amplification and primer-dimers. The presence of UNG and dUTP’s eliminates carryover contamination, the thermolabile UNG is active at room temperature before being completely and irreversibly inactivated when heated to 95°C, this means your PCR product is suitable for downstream processing.
MegaMix Emerald using MicroGreen dye ensures high sensitivity and earlier CTs than competitor mixes. When combined with Microzone’s SwiftScript cDNA synthesis product, you achieve the perfect combination for RT-qPCR too.
Ultra Fast PCR – MegaMix Emerald is capable to powering your PCR to new heights. With 5” denaturation 1” annealing a complete PCR can be completed in just over 30 minutes. You can download our full application paper here!
The product is stable at -20°C, 4°C and RT for various time lengths.
Additional information
| Quantity | 100 Reactions, 200 Reactions, 1000 Reactions |
|---|---|
| ROX | No-ROX, Lo-ROX, Hi-ROX |
MegaMix Emerald dye based qPCR Mastermix
Containing all the components needed to perform dye based qPCR swiftly and reliably. The 2X mix contains chemically modified Hot Start Taq DNA polymerase, intercalating dye, dUTP and UNG in enhancing Microzone's proprietary PCR buffer optimised for amplifying low copy DNA targets.
We have incorporated MicroGREEN Dye ( a third generation intercalating highly fluorescent dye that binds to double stranded DNA) in to MegaMix Emerald. This dye mix combinations makes MegaMix Emerald the perfect choice for qPCR, Melt Curve Analysis and High Resolution Melting (HRM).
The Hot Start Taq polymerase is chemically inactivated until heating to 95°C, providing excellent sensitivity and specificity; eliminating the formation of non-specific amplification and primer-dimers. The presence of UNG and dUTP’s eliminates carryover contamination, the thermolabile UNG is active at room temperature before being completely and irreversibly inactivated when heated to 95°C, this means your PCR product is suitable for downstream processing.
MegaMix Emerald using MicroGreen dye ensures high sensitivity and earlier CTs than competitor mixes. When combined with Microzone's SwiftScript cDNA synthesis product, you achieve the perfect combination for RT-qPCR too.
Ultra Fast PCR - MegaMix Emerald is capable to powering your PCR to new heights. With 5'' denaturation 1'' annealing a complete PCR can be completed in just over 30 minutes. You can download our full application paper here!
The product is stable at -20°C, 4°C and RT for various time lengths.
MegaMix Emerald Demonstrates Excellent Reproducibility and Linearity When Amplifying from Saliva gDNA. MegaMix Emerald qPCR amplification of hp53 from serially diluted saliva gDNA extracted using Microzone Blood Tissue Saliva Extraction Kit.
- Incorporates Microzone’s proprietary third generation, non-inhibitory intercalating dye, microGREEN, for increased fluorescence and unmatched sensitivity.
- Eliminates carry over contamination—incorporation of thermolabile UNG and dUTP prevent amplicon contamination from previous runs without degrading generated amplicons.
- Compatible with any qPCR instrument with a FAM/SYBR channel.
- Specificity—Hot Start Taq DNA Polymerase in optimized buffer eliminates non-specific amplification and the formation of primer dimers.
- Sensitivity—Single copy detection.
- Versatile— compatible with standard and fast cycling conditions, GC/AT rich templates.
- Reproducibility and convenience—ready to use 2X format.
- Inhibitor Resistance—suitable for direct to PCR with products such as microLYSIS-Plus and microLYSIS-RNA.
- Third generation intercalating dye— no inhibition of PCR, even at high concentrations, suitable for High Resolution Melting.
- Dye based qPCR
- Use with gDNA, Viral DNA, cDNA….
- Use for quantification, gene expression, SNP detection using High Resolution Melting (HRM).
MegaMix Emerald qPCR with UNG (orange) exhibits earlier Cq values and superior sensitivity vs competitor A (black), when amplifying the RNase P gene from a 6X, 1 in 10 serial dilution of 1 µg human DNA. BMS MIC.
- Incorporates Microzone’s proprietary third generation, non-inhibitory intercalating dye, microGREEN, for increased fluorescence and unmatched sensitivity.
- Eliminates carry over contamination—incorporation of thermolabile UNG and dUTP prevent amplicon contamination from previous runs without degrading generated amplicons.
- Compatible with any qPCR instrument with a FAM/SYBR channel.
- Specificity—Hot Start Taq DNA Polymerase in optimized buffer eliminates non-specific amplification and the formation of primer dimers.
- Sensitivity—Single copy detection.
- Versatile— compatible with standard and fast cycling conditions, GC/AT rich templates.
- Reproducibility and convenience—ready to use 2X format.
- Inhibitor Resistance—suitable for direct to PCR with products such as microLYSIS-Plus and microLYSIS-RNA.
- Third generation intercalating dye— no inhibition of PCR, even at high concentrations, suitable for High Resolution Melting.
- Dye based qPCR
- Use with gDNA, Viral DNA, cDNA….
- Use for quantification, gene expression, SNP detection using High Resolution Melting (HRM).
MegaMix Emerald qPCR with UNG (orange) exhibits earlier Cq values and superior sensitivity vs competitor A (black), when amplifying the RNase P gene from a 6X, 1 in 10 serial dilution of 1 µg human DNA. BMS MIC.
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