PCR Testing in the Field

Field-based PCR testing is increasingly essential for applications such as environmental monitoring, infectious disease diagnostics, and point-of-care testing. Performing PCR outside traditional laboratory settings requires reagents and workflows optimized for portability, robustness, and reliability under challenging conditions. Microzone provides innovative solutions for field-based PCR, including its Lyophilised Range of mastermixes, highly stable liquid formulations, and direct-to-PCR lysis buffers, ensuring consistent and reliable results in diverse environments.

Direct-to-PCR Lysis Buffers

Microzone’s direct-to-PCR lysis buffers, such as microLYSIS Plus and microLYSIS RNA, are ideal for field testing as they simplify sample preparation by eliminating the need for extensive equipment or time-consuming protocols. These buffers efficiently release DNA or RNA from crude samples through a straightforward thermal lysis process, making them particularly suitable for field applications where resources are limited. This reduces handling steps and accelerates workflows, enabling rapid PCR testing directly from samples such as tissue, blood, or environmental swabs.

Mastermixes for Field Applications

Microzone offers both lyophilized and high-stability liquid mastermixes designed to meet the demands of field-based testing.

· Lyophilised Mastermixes: The Lyophilised MegaMix Emerald Dye qPCR Mastermix and Lyophilised MegaMix Platinum Probe qPCR Mastermix are pre-mixed, shelf-stable, and require no refrigeration. These reagents can be reconstituted on-site with water or buffer, making them highly portable and perfect for remote or resource-limited settings.

MegaMix Emerald is optimized for dye-based qPCR assays, offering robust amplification and clear results.

MegaMix Platinum is ideal for probe-based qPCR assays, ensuring high sensitivity and specificity.

High-Stability Liquid Mastermixes: For applications requiring liquid reagents, Microzone’s liquid mastermixes are engineered for exceptional thermal stability, some maintaining performance for over 40 days at 40°C. These formulations are well-suited for regions with limited refrigeration or variable storage conditions, ensuring reliable performance across diverse field environments.

Applications

Microzone’s solutions are suited to a wide range of field-based PCR applications, including:

• Pathogen detection in environmental and agricultural samples.
• Field diagnostics for infectious diseases.
• Wildlife and veterinary monitoring.
• Genetic marker analysis in remote locations.

Empowering Reliable Field-Based Testing

With a complete range of solutions, including direct-to-PCR lysis buffers, lyophilized mastermixes, and stable liquid formulations, Microzone simplifies and enhances field PCR workflows. By reducing equipment needs and streamlining sample preparation, these products enable accurate and efficient testing in even the most demanding conditions.