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2MMC
MegaMix Crystal Q5 High Fidelity
£115.00 – £780.00
Description
The 2X MegaMix Crystal Q5 High Fidelity Mastermix is a high-performance solution for researchers requiring accurate and reliable DNA amplification. This mastermix combines Q5 High Fidelity DNA Polymerase with an enhanced buffer containing balanced dNTPs, optimal Mg2+, and proprietary stabilizers to support challenging PCR applications. Designed to reduce uncertainty, it simplifies the setup process while ensuring reproducible and consistent results.
The Q5 High Fidelity DNA Polymerase offers significantly improved fidelity (280X) compared to standard enzymes, making it well-suited for applications such as cloning, sequencing, and amplifying complex or GC-rich templates. The mastermix includes a Hot-Start mechanism for better specificity and sensitivity, enabling reaction setup at room temperature. With support for long amplicons up to 40 kb and faster extension times, it is an ideal choice for demanding workflows like next-generation sequencing (NGS), site-directed mutagenesis, and amplification of difficult templates.
For added versatility, the product is compatible with Microzone’s optional 4X microFORCE buffer to further enhance performance on challenging samples. The Red Mastermix variant also includes an inert loading dye to simplify gel electrophoresis workflows without interfering with downstream applications.
MegaMix – by Microzone
Microzone’s MegaMix range of PCR mastermixes covers a broad range of a scientist’s requirements. We offer a MegaMix for most occasions. You can see our range here.
Additional information
| Type | MegaMix Crystal Q5 High Fidelity DNA Mastermix, MegaMix Crystal Q5 High Fidelity DNA Polymerase, MegaMix Crystal Q5 High Fidelity Red Mastermix |
|---|---|
| Quantity | 100 reactions, 500 reactions, 100 units, 500 units |
The 2X MegaMix Crystal Q5 High Fidelity Mastermix is a high-performance solution for researchers requiring accurate and reliable DNA amplification. This mastermix combines Q5 High Fidelity DNA Polymerase with an enhanced buffer containing balanced dNTPs, optimal Mg2+, and proprietary stabilizers to support challenging PCR applications. Designed to reduce uncertainty, it simplifies the setup process while ensuring reproducible and consistent results.
The Q5 High Fidelity DNA Polymerase offers significantly improved fidelity (280X) compared to standard enzymes, making it well-suited for applications such as cloning, sequencing, and amplifying complex or GC-rich templates. The mastermix includes a Hot-Start mechanism for better specificity and sensitivity, enabling reaction setup at room temperature. With support for long amplicons up to 40 kb and faster extension times, it is an ideal choice for demanding workflows like next-generation sequencing (NGS), site-directed mutagenesis, and amplification of difficult templates.
For added versatility, the product is compatible with Microzone's optional 4X microFORCE buffer to further enhance performance on challenging samples. The Red Mastermix variant also includes an inert loading dye to simplify gel electrophoresis workflows without interfering with downstream applications.
MegaMix - by Microzone
Microzone's MegaMix range of PCR mastermixes covers a broad range of a scientist's requirements. We offer a MegaMix for most occasions. You can see our range here.
Superior amplicon yield using MegaMix Crystal Q5 High Fidelity Polymerase vs commercially available Fusion polymerases. Gel image shows amplification of 8.5 kb fragment from human gDNA, using template concentrations of 50, 100 and 200 ng. PCR was performed in 50 µL reaction using Microzone’s MegaMix Crystal Q5 High-Fidelity range. M: 1 kb DNA Ladder.
- 280X higher fidelity compared to Taq Polymerase.
- Hot-Start mechanism for enhanced specificity and sensitivity.
- Suitable for amplicons up to 40 kb.
- Ultra-low error rate with 3′-5′ exonuclease proofreading activity.
- Optional red dye for easy gel loading (Red Mastermix only).
- Produces blunt-ended PCR products suitable for cloning.
- Long-range PCR amplifications (up to 40 kb).
- NGS library preparation and sequencing.
- Site-directed mutagenesis.
- Amplification of challenging (GC-rich) templates.
License
This product is covered by one or more patents.
This product is licensed for research and commercial use from Bio-Rad Laboratories, Inc., under U.S. Pat. Nos. 6,627,424, 7,541,170, 7,670,808, 7,666,645, and corresponding patents in other countries. No rights are granted for use of the product for Digital PCR or real-time PCR applications, with the exception of quantification in Next Generation Sequencing workflows.
- Polymerase: Q5 High Fidelity DNA Polymerase.
- Buffer: Enhanced with stabilizers, optimal Mg2+, and dNTPs.
- Formats: Available with or without red loading dye.
- Recommended Reaction Setup:
- Initial Denaturation: 98°C, 30s
- Cycling (25-35 cycles):
- Denaturation: 98°C, 5-10s
- Annealing: 60-68°C, 10-15s
- Extension: 72°C, 30s/kb
- Final Elongation: 72°C, 2min
- 280X higher fidelity compared to Taq Polymerase.
- Hot-Start mechanism for enhanced specificity and sensitivity.
- Suitable for amplicons up to 40 kb.
- Ultra-low error rate with 3′-5′ exonuclease proofreading activity.
- Optional red dye for easy gel loading (Red Mastermix only).
- Produces blunt-ended PCR products suitable for cloning.
- Long-range PCR amplifications (up to 40 kb).
- NGS library preparation and sequencing.
- Site-directed mutagenesis.
- Amplification of challenging (GC-rich) templates.
License
This product is covered by one or more patents.
This product is licensed for research and commercial use from Bio-Rad Laboratories, Inc., under U.S. Pat. Nos. 6,627,424, 7,541,170, 7,670,808, 7,666,645, and corresponding patents in other countries. No rights are granted for use of the product for Digital PCR or real-time PCR applications, with the exception of quantification in Next Generation Sequencing workflows.
- Polymerase: Q5 High Fidelity DNA Polymerase.
- Buffer: Enhanced with stabilizers, optimal Mg2+, and dNTPs.
- Formats: Available with or without red loading dye.
- Recommended Reaction Setup:
- Initial Denaturation: 98°C, 30s
- Cycling (25-35 cycles):
- Denaturation: 98°C, 5-10s
- Annealing: 60-68°C, 10-15s
- Extension: 72°C, 30s/kb
- Final Elongation: 72°C, 2min
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Order before 12:00 GMT, dispatched today.
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Custom Orders Made Easy
At Microzone, we recognise the unique demands of every laboratory. Whether you're ramping up experiments or seeking specialised formulations, our dedicated team is on standby. Contact us today and benefit from personalised attention that ensures your order is precisely tailored.
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