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2C2S-50
Cell2Seq – 16S rRNA Microbial Identification Kit
£225.00
Description
Part of the ‘Free From’ Microzone range, the Cell2Seq – 16S rRNA Microbial Identification Kit is a cell-to-sequence solution designed to streamline and simplify the 16S Sanger sequencing workflow.
This innovative kit provides all the necessary components for the three critical steps for sample preparation for sequencing: microbial lysis, high-fidelity PCR amplification, and post-PCR clean-up. Microbial lysis is achieved with microLYSIS Plus, a reagent formulated for efficient microbial lysis and high-quality DNA extraction through a rapid thermal lysis protocol.
The whole process takes place in a single tube and does not involve the use of solvents or other harmful chemicals usually required in traditional DNA extraction methods. High-fidelity PCR amplification is performed using the 2X MegaMix Crystal Q5 High Fidelity Mastermix, which consists of Q5 DNA Polymerase in an enhanced buffer with optimal Mg2+ concentration, balanced dNTPs, and a proprietary formulation of stabilizers and enhancers specifically optimized for our enzyme. Q5 High Fidelity DNA Polymerase offers unmatched accuracy with a 280-fold higher fidelity compared to Taq Polymerase and a 5-fold higher fidelity compared to standard Pfu Polymerase. This enzyme features a Hot-Start mechanism for increased specificity and sensitivity, allowing for room temperature reaction setup. Post-PCR clean-up utilizes microCLEAN reagent, which requires less than 15 minutes to efficiently remove unwanted residual dNTPs, primer-dimers, enzymes, and unincorporated dyes from PCR products while maximizing amplicon recovery for high-quality sequencing.
It is a quick and simple protocol with minimal requirements for equipment and plasticware, significantly reducing the environmental impact of the process.
License
This product is covered by one or more patents.
This product is licensed for research and commercial use from Bio-Rad Laboratories, Inc., under U.S. Pat. Nos. 6,627,424, 7,541,170, 7,670,808, 7,666,645, and corresponding patents in other countries. No rights are granted for use of the product for Digital PCR or real-time PCR applications, with the exception of quantification in Next Generation Sequencing workflows.
Additional information
| Quantity | 50 reactions |
|---|
Part of the ‘Free From’ Microzone range, the Cell2Seq – 16S rRNA Microbial Identification Kit is a cell-to-sequence solution designed to streamline and simplify the 16S Sanger sequencing workflow.
This innovative kit provides all the necessary components for the three critical steps for sample preparation for sequencing: microbial lysis, high-fidelity PCR amplification, and post-PCR clean-up. Microbial lysis is achieved with microLYSIS Plus, a reagent formulated for efficient microbial lysis and high-quality DNA extraction through a rapid thermal lysis protocol.
The whole process takes place in a single tube and does not involve the use of solvents or other harmful chemicals usually required in traditional DNA extraction methods. High-fidelity PCR amplification is performed using the 2X MegaMix Crystal Q5 High Fidelity Mastermix, which consists of Q5 DNA Polymerase in an enhanced buffer with optimal Mg2+ concentration, balanced dNTPs, and a proprietary formulation of stabilizers and enhancers specifically optimized for our enzyme. Q5 High Fidelity DNA Polymerase offers unmatched accuracy with a 280-fold higher fidelity compared to Taq Polymerase and a 5-fold higher fidelity compared to standard Pfu Polymerase. This enzyme features a Hot-Start mechanism for increased specificity and sensitivity, allowing for room temperature reaction setup. Post-PCR clean-up utilizes microCLEAN reagent, which requires less than 15 minutes to efficiently remove unwanted residual dNTPs, primer-dimers, enzymes, and unincorporated dyes from PCR products while maximizing amplicon recovery for high-quality sequencing.
It is a quick and simple protocol with minimal requirements for equipment and plasticware, significantly reducing the environmental impact of the process.
License
This product is covered by one or more patents.
This product is licensed for research and commercial use from Bio-Rad Laboratories, Inc., under U.S. Pat. Nos. 6,627,424, 7,541,170, 7,670,808, 7,666,645, and corresponding patents in other countries. No rights are granted for use of the product for Digital PCR or real-time PCR applications, with the exception of quantification in Next Generation Sequencing workflows.
Successful amplification from bacterial lysates in microLYSIS Plus and no sample loss after PCR clean-up step using Cell2Seq – 16S rRNA Microbial Identification Kit. V3-V4 regions of 16S rRNA gene amplified with 2X MegaMix Crystal Q5 HiFi MasterMix: before (-) or after PCR clean-up with microCLEAN (+). Solid arrows show the presence of PCR residues; Dashed arrows show removal of PCR residues after microCLEAN PCR clean-up. M = 100 bp DNA
Excellent quality metrics obtained from 16S sequencing using Cell2Seq – 16S rRNA Microbial Identification Kit. Different bacteria species prepared in microLYSIS Plus using either a bacterial colony or broth (OD of 0.1 or OD 1), 16S amplification was performed with MegaMix Crystal Q5 High-Fidelity MasterMix and PCR clean-up was achieved with microCLEAN. Graphs show the contiguous read length (A), and Quality Score (B) obtained from Sanger sequencing.
- Combines microbial lysis, high-fidelity PCR amplification, and post-PCR clean-up in one kit
- Reduced processing time without compromising sequencing results
- Environmentally conscious workflow with minimal equipment needs
- Accurate and reliable microbial identification using Sanger sequencing
- Sanger sequencing sample preparation: microbial lysis, high-fidelity amplification, and PCR clean-up
- Classifying microorganisms, constructing phylogenetic trees, detecting novel species, and more
- Contains microLYSIS Plus, 2X MegaMix Crystal Q5 High Fidelity Mastermix, 16S rRNA Primer Mix, and microCLEAN PCR clean-up reagent
- Optimized for 16S rRNA gene amplification and sequencing
- Suitable for bacterial lysates, including colony and broth samples
- Combines microbial lysis, high-fidelity PCR amplification, and post-PCR clean-up in one kit
- Reduced processing time without compromising sequencing results
- Environmentally conscious workflow with minimal equipment needs
- Accurate and reliable microbial identification using Sanger sequencing
- Sanger sequencing sample preparation: microbial lysis, high-fidelity amplification, and PCR clean-up
- Classifying microorganisms, constructing phylogenetic trees, detecting novel species, and more
- Contains microLYSIS Plus, 2X MegaMix Crystal Q5 High Fidelity Mastermix, 16S rRNA Primer Mix, and microCLEAN PCR clean-up reagent
- Optimized for 16S rRNA gene amplification and sequencing
- Suitable for bacterial lysates, including colony and broth samples
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