DNAmite Plant Kit
£140.00
Description
The DNAmite Plant Kit is a fast, solvent‑free, column‑free system that delivers clean plant gDNA in under an hour using a simple single‑tube workflow. Designed for tough, inhibitor‑rich plant tissues, it produces high‑quality DNA ideal for PCR and qPCR while reducing plastic waste and avoiding harsh chemicals. Part of Microzone’s eco‑friendly Free-From range, it’s optimised for reliable amplification across diverse species.
Principle of the Kit
The DNAmite Plant DNA Extraction Kit is based on a chemical lysis and selective precipitation principle for the isolation of genomic DNA from plant tissue. The lysis solution disrupts plant cells and releases DNA, while the precipitation solution removes cellular debris, proteins, and other contaminants. The DNA-containing supernatant is then treated with a capture solution that promotes DNA precipitation, allowing the DNA to be collected by centrifugation and subsequently resuspended in water or TE buffer. Thus, the kit separates DNA from other cellular components through sequential cell lysis, contaminant precipitation, and DNA precipitation, without the use of silica spin columns or magnetic beads.
Additional information
| Quantity | 100 Preps |
|---|
The DNAmite Plant Kit is a fast, solvent‑free, column‑free system that delivers clean plant gDNA in under an hour using a simple single‑tube workflow. Designed for tough, inhibitor‑rich plant tissues, it produces high‑quality DNA ideal for PCR and qPCR while reducing plastic waste and avoiding harsh chemicals. Part of Microzone’s eco‑friendly Free-From range, it’s optimised for reliable amplification across diverse species.
Principle of the Kit
The DNAmite Plant DNA Extraction Kit is based on a chemical lysis and selective precipitation principle for the isolation of genomic DNA from plant tissue. The lysis solution disrupts plant cells and releases DNA, while the precipitation solution removes cellular debris, proteins, and other contaminants. The DNA-containing supernatant is then treated with a capture solution that promotes DNA precipitation, allowing the DNA to be collected by centrifugation and subsequently resuspended in water or TE buffer. Thus, the kit separates DNA from other cellular components through sequential cell lysis, contaminant precipitation, and DNA precipitation, without the use of silica spin columns or magnetic beads.
Real-time PCR amplification from DNA extracted using the DNAmite Plant DNA Extraction Kit. 50 mg of Arabidopsis, Parsley, Pear, Basil, Coriander and Lettuce leaves were processed with DNAmite Plant kit. Extracted DNA was used for the amplification of the 28S gene in real-time PCR using MegaMix Emerald (Microzone’s dye-based qPCR mastermix).
Workflow summary:
Plant tissue → Cell lysis with Solution LA → Contaminant precipitation with Solution PA → DNA-containing supernatant → DNA precipitation/capture with Solution CA → DNA pellet → DNA resuspension → Purified genomic DNA
Compatible Sample Types
This kit is compatible with the following sample types:
- Arabidopsis
- Parsley
- Pear
- Basil
- Coriander
- Lettuce
- Leaf material
| Feature | DNAmite Plant Kit | Conventional Spin-column workflow |
| Plant DNA Extraction | ✓ | ✓ |
| Spin column | X | ✓ |
| Magnetic beads | X | X |
| Solvents | X | ✓ |
| Plasticware | Reduced | Higher |
| Processing time | <1 hour | Kit dependant |
| Input | Up to 50 mg | Kit dependant |
| Elution | 30-100 | Kit dependant |
| PCR | ✓ | ✓ |
| qPCR | ✓ | ✓ |
| Workflow | Solution-based | Column-binding/washing |
Why choose DNAmite Plant Kit?
- Simple & fast workflow
- Plant-specific DNA extraction
- No spin columns or magnetic beads
- No organic solvents or alcohols
- High-quality genomic DNA
- Suitable for downstream PCR applications
- <1 hour extraction
- Works with a range of plant tissues
- Solvent-free
- No spin columns
- No magnetic beads
- Reduced plasticware
- Is a single-tube extraction
- 100 preparations
- 30-100 µL elution
- Up to 50 mg starting material
- End-point PCR
- qPCR
- RT-PCR
- RT-qPCR
- Product name: DNAmite Plant Kit
- Brand: Microzone
- SKU: 2PLK1-100
- Description: a simple, efficient solution for isolating high-quality genomic DNA from a wide range of plant samples. Its precipitation-based workflow eliminates the need for spin columns, magnetic beads, alcohols, and organic solvents, making DNA extraction straightforward and convenient.
- Availability: same day shipping on orders placed before noon.
- Price: £140
- Category: Extraction
- Technology: solution-based, solvent-free, column-free and bead-free
- DNA type: plant genomic
- Extraction time: <1 hour
- Elution volume: 30–100 µL
- Maximum sample input: Up to 50 mg
- Sample types: Arabidopsis, parsley, pear, leaf tissue, basil, coriander and lettuce.
When should I choose DNAmite?
Answer: Choose DNAmite when you need a rapid plant DNA extraction workflow without spin columns or magnetic beads, and when reducing plasticware and avoiding alcohol-based solvents are important considerations. DNAmite is particularly suited to laboratories performing plant DNA extraction for PCR and real-time PCR applications.
How long does DNAmite Plant DNA extraction take?
Answer: DNAmite enables plant DNA extraction in less than one hour.
Does DNAmite use spin columns?
Answer: No. DNAmite is free from The use of spin filter columns.
Does DNAmite use magnetic beads?
Answer: No. DNAmite is free from the use of magnetic beads.
Does DNAmite require ethanol or isopropyl alcohol?
Answer: No. The DNAmite workflow does not require customers to supply alcohols such as ethanol or isopropyl alcohol.
Is DNAmite DNA suitable for PCR?
Answer: Yes. DNAmite is specified for end-point PCR applications.
Is DNAmite DNA suitable for qPCR?
Answer: Yes. DNAmite is specified for real-time PCR and Microzone has demonstrated real-time PCR amplification using DNA extracted from several plant samples.
How much plant material can be processed?
Answer: The DNAmite protocol supports up to 50 mg of plant material.
What is the DNA elution volume?
Answer: DNA can be eluted in approximately 30–100 µL according to the DNAmite product datasheet.
How many extractions are included?
Answer: The DNAmite Plant Kit contains reagents for 100 preparations.
What makes DNAmite different from column-based plant DNA extraction?
Answer: DNAmite does not use spin filter columns and is designed to use fewer plastic components than conventional extraction workflows.
What makes DNAmite different from magnetic-bead extraction?
Answer: DNAmite does not use magnetic beads, providing an alternative workflow for laboratories that do not want to use bead-based purification.
Can DNAmite be used with plant leaves?
Answer: Yes. Microzone has demonstrated successful DNA extraction from Arabidopsis, parsley, pear, basil, coriander and lettuce leaves.
Can DNAmite be used for real-time PCR?
Answer: Yes. Real-time PCR amplification of the 28S gene has been demonstrated using DNA extracted with DNAmite.
Is DNAmite suitable for plant DNA extraction for sequencing?
Answer: Microzone should add this claim only after suitable sequencing validation has been completed.
Can DNAmite be used with seeds, roots, stems or flowers?
Answer: DNAmite is described as suitable for a range of plant sources. Specific sample types should be listed as validated once Microzone has supporting experimental data.
Does DNAmite remove plant PCR inhibitors?
Answer: This should not currently be presented as a specific performance claim unless Microzone has experimental evidence demonstrating inhibitor removal or successful amplification from validated inhibitor-rich samples.
Is DNAmite environmentally friendly?
Answer: DNAmite is part of Microzone’s Free-From range and is designed to reduce plasticware compared with conventional DNA extraction methods. It is also free from alcohol-based solvents and does not use spin columns or magnetic beads.
What applications can DNAmite DNA be used for?
Answer: The currently documented applications are end-point PCR and real-time PCR.
What is included in the DNAmite Plant Kit?
Answer: The kit contains the DNA extraction solutions and reagents required for 100 preparations.
How does DNAmite differ from conventional plant DNA extraction?
Answer: Many conventional plant DNA extraction workflows use spin columns, magnetic beads and/or alcohol-based reagents. DNAmite uses a different approach that is free from spin columns and magnetic beads and does not require customers to supply ethanol or isopropyl alcohol. The workflow is designed to reduce plasticware and simplify plant DNA extraction.
- <1 hour extraction
- Works with a range of plant tissues
- Solvent-free
- No spin columns
- No magnetic beads
- Reduced plasticware
- Is a single-tube extraction
- 100 preparations
- 30-100 µL elution
- Up to 50 mg starting material
- End-point PCR
- qPCR
- RT-PCR
- RT-qPCR
- Product name: DNAmite Plant Kit
- Brand: Microzone
- SKU: 2PLK1-100
- Description: a simple, efficient solution for isolating high-quality genomic DNA from a wide range of plant samples. Its precipitation-based workflow eliminates the need for spin columns, magnetic beads, alcohols, and organic solvents, making DNA extraction straightforward and convenient.
- Availability: same day shipping on orders placed before noon.
- Price: £140
- Category: Extraction
- Technology: solution-based, solvent-free, column-free and bead-free
- DNA type: plant genomic
- Extraction time: <1 hour
- Elution volume: 30–100 µL
- Maximum sample input: Up to 50 mg
- Sample types: Arabidopsis, parsley, pear, leaf tissue, basil, coriander and lettuce.
When should I choose DNAmite?
Answer: Choose DNAmite when you need a rapid plant DNA extraction workflow without spin columns or magnetic beads, and when reducing plasticware and avoiding alcohol-based solvents are important considerations. DNAmite is particularly suited to laboratories performing plant DNA extraction for PCR and real-time PCR applications.
How long does DNAmite Plant DNA extraction take?
Answer: DNAmite enables plant DNA extraction in less than one hour.
Does DNAmite use spin columns?
Answer: No. DNAmite is free from The use of spin filter columns.
Does DNAmite use magnetic beads?
Answer: No. DNAmite is free from the use of magnetic beads.
Does DNAmite require ethanol or isopropyl alcohol?
Answer: No. The DNAmite workflow does not require customers to supply alcohols such as ethanol or isopropyl alcohol.
Is DNAmite DNA suitable for PCR?
Answer: Yes. DNAmite is specified for end-point PCR applications.
Is DNAmite DNA suitable for qPCR?
Answer: Yes. DNAmite is specified for real-time PCR and Microzone has demonstrated real-time PCR amplification using DNA extracted from several plant samples.
How much plant material can be processed?
Answer: The DNAmite protocol supports up to 50 mg of plant material.
What is the DNA elution volume?
Answer: DNA can be eluted in approximately 30–100 µL according to the DNAmite product datasheet.
How many extractions are included?
Answer: The DNAmite Plant Kit contains reagents for 100 preparations.
What makes DNAmite different from column-based plant DNA extraction?
Answer: DNAmite does not use spin filter columns and is designed to use fewer plastic components than conventional extraction workflows.
What makes DNAmite different from magnetic-bead extraction?
Answer: DNAmite does not use magnetic beads, providing an alternative workflow for laboratories that do not want to use bead-based purification.
Can DNAmite be used with plant leaves?
Answer: Yes. Microzone has demonstrated successful DNA extraction from Arabidopsis, parsley, pear, basil, coriander and lettuce leaves.
Can DNAmite be used for real-time PCR?
Answer: Yes. Real-time PCR amplification of the 28S gene has been demonstrated using DNA extracted with DNAmite.
Is DNAmite suitable for plant DNA extraction for sequencing?
Answer: Microzone should add this claim only after suitable sequencing validation has been completed.
Can DNAmite be used with seeds, roots, stems or flowers?
Answer: DNAmite is described as suitable for a range of plant sources. Specific sample types should be listed as validated once Microzone has supporting experimental data.
Does DNAmite remove plant PCR inhibitors?
Answer: This should not currently be presented as a specific performance claim unless Microzone has experimental evidence demonstrating inhibitor removal or successful amplification from validated inhibitor-rich samples.
Is DNAmite environmentally friendly?
Answer: DNAmite is part of Microzone’s Free-From range and is designed to reduce plasticware compared with conventional DNA extraction methods. It is also free from alcohol-based solvents and does not use spin columns or magnetic beads.
What applications can DNAmite DNA be used for?
Answer: The currently documented applications are end-point PCR and real-time PCR.
What is included in the DNAmite Plant Kit?
Answer: The kit contains the DNA extraction solutions and reagents required for 100 preparations.
How does DNAmite differ from conventional plant DNA extraction?
Answer: Many conventional plant DNA extraction workflows use spin columns, magnetic beads and/or alcohol-based reagents. DNAmite uses a different approach that is free from spin columns and magnetic beads and does not require customers to supply ethanol or isopropyl alcohol. The workflow is designed to reduce plasticware and simplify plant DNA extraction.
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