MAGneat FFPE DNA Extraction Kit

What is the MAGneat FFPE DNA Extraction Kit?
Answer: The MAGneat FFPE DNA Extraction Kit is a magnetic bead-based system designed to isolate high-quality genomic DNA from formalin-fixed, paraffin-embedded (FFPE) tissue. The workflow combines deparaffinisation, Proteinase K digestion, crosslink reversal and magnetic bead purification to produce DNA suitable for a range of downstream molecular biology applications.

What are FFPE tissue samples?
Answer: FFPE (formalin-fixed, paraffin-embedded) tissue samples are biological specimens preserved by formalin fixation and embedding in paraffin wax. This method is widely used in pathology and research because it preserves tissue morphology and enables long-term sample storage.

Why is FFPE DNA difficult to extract?
Answer: Formalin fixation can cause DNA fragmentation and chemical crosslinking between DNA and proteins. Paraffin must also be removed before purification. These factors make the recovery of high-quality DNA from FFPE tissue more challenging than from fresh or frozen samples.

How does the kit remove paraffin?
Answer: The MAGneat FFPE DNA Extraction Kit includes a dedicated Xylene free deparaffinisation step before DNA purification. Removing the paraffin enables more effective tissue digestion and improves access to the DNA during extraction.

Does the kit reverse formalin crosslinks?
Answer: Yes. The extraction workflow includes conditions designed to reverse formalin-induced crosslinks, helping release genomic DNA for subsequent purification and downstream analysis.

Is the DNA suitable for PCR?
Answer: Yes. The kit produces purified genomic DNA suitable for PCR and other sensitive enzymatic applications.

Is the DNA suitable for qPCR?
Answer: Yes. Extracted DNA is suitable for quantitative PCR (qPCR) workflows, including applications requiring DNA isolated from FFPE tissue.

Can the DNA be used for Next Generation Sequencing (NGS)?
Answer: Yes. DNA extracted using the MAGneat FFPE DNA Extraction Kit is suitable for Next Generation Sequencing (NGS) workflows following appropriate library preparation.

Is the DNA suitable for Sanger sequencing?
Answer: Yes. Purified DNA can be used for Sanger sequencing following appropriate downstream sample preparation.

Can the kit be used for mutation analysis?
Answer: Yes. DNA extracted from FFPE tissue can be used for mutation analysis and other molecular genetics applications.

Is the kit suitable for biomarker research?
Answer: Yes. The MAGneat FFPE DNA Extraction Kit produces purified genomic DNA suitable for biomarker research and other translational research applications using FFPE tissue.

Can archived FFPE samples be processed?
Answer: Yes. The kit can be used to extract genomic DNA from archived FFPE tissue. DNA yield and quality may vary depending on factors such as sample age, fixation conditions and storage history.

Can the workflow be automated?
Answer: Yes. The MAGneat FFPE DNA Extraction Kit is designed for both manual and automated magnetic bead-based workflows, providing flexibility for different laboratory throughput requirements.

Which automation platforms are supported?
Answer: MAGneat is designed for automation and has been validated across multiple magnetic bead-based extraction platforms, including the KingFisher® Purification System and the HeiDi-NA automated magnetic bead processing system. The HeiDi-NA processes up to 16 samples per run.

What equipment and additional reagents are required?
Answer: Typical equipment includes a microtome, magnetic rack or compatible automated magnetic processor, microcentrifuge tubes, pipettes with appropriate tips, and a heating block capable of reaching 90°C. Additional reagents include molecular-grade water, 100% ethanol and 100% isopropanol. RNase A may be used when RNA removal is required. Refer to the IFU for the full list of required materials and sample preparation requirements.

How long does the extraction take?
Answer: The MAGneat FFPE DNA extraction workflow can be completed in approximately 2-3 hours, although total processing time may vary depending on sample preparation and workflow conditions.

How should the kit be stored?
Answer: All kit components can be stored at room temperature, except the MAGneat magnetic beads, which should be stored at 2–8°C to prevent microbial growth.

What downstream applications are supported?
Answer: Purified genomic DNA is suitable for a range of downstream applications, including PCR, qPCR, Next Generation Sequencing (NGS), Sanger sequencing, mutation analysis, biomarker research and other molecular biology workflows.

How does magnetic bead extraction compare with spin-column FFPE extraction?
Answer: Magnetic bead-based extraction offers an automation-friendly alternative to traditional spin-column workflows and can reduce manual handling. The MAGneat FFPE DNA Extraction Kit combines deparaffinisation, crosslink reversal and magnetic bead purification in a workflow specifically designed for FFPE tissue.

Why choose the MAGneat FFPE DNA Extraction Kit?
Answer: The MAGneat FFPE DNA Extraction Kit combines optimised FFPE sample preparation with magnetic bead purification and automation compatibility. It is designed to recover high-quality genomic DNA suitable for applications including PCR, qPCR, NGS, mutation analysis and biomarker research, providing a flexible solution for pathology, oncology and molecular research workflows.