Absolute Quantification

Absolute quantification is a precise method for determining the exact copy number or concentration of a DNA or RNA target within a sample. This technique is widely used in applications such as viral load determination, plasmid quantification, and gene expression studies where accuracy and reliability are essential.

Microzone offers a comprehensive range of reagents for absolute quantification. For DNA detection, MegaMix Platinum Probe qPCR and MegaMix Emerald Dye qPCR deliver robust, high-sensitivity amplification using probe-based or dye-based methods, respectively. For RNA workflows, MegaMix Platinum Probe RT-qPCR and MegaMix Emerald Dye RT-qPCR combine efficient reverse transcription with qPCR, ensuring accurate and reliable quantification. Additionally, the SwiftScript cDNA Synthesis Kit supports two-step methods, producing high-quality cDNA compatible with downstream probe- or dye-based qPCR.

Designed for compatibility with standard curves and reference standards, Microzone’s advanced formulations minimize variability and maximize sensitivity, ensuring precise quantification even for low-abundance or complex samples.

Achieve reliable and accurate absolute quantification with Microzone’s innovative solutions.